Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
NAD+ | pH 8.0, 25°C | Bacillus subtilis | |
8.7 | - |
beta-D-glucose | pH 8.0, 25°C | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-Sepharose, Blue Fractogel | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus subtilis |
Storage Stability | Organism |
---|---|
0°C, 67 mM KH2PO4, pH 6.5, 0.5 M NaCl, stable for a few months | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + NAD+ | - |
Bacillus subtilis | D-glucono-1,5-lactone + NADH + H+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
shifting the pH from 6.5 to 9.0 leads to inactivation, reactivation to 100% activity by shifting pH back to 6.5, protein concentration higher than 0.1 mg/ml | Bacillus subtilis |
9 | - |
inactivation above, Gluc-DH-S | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus subtilis |