Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, isoenzymes III and IV | Priestia megaterium |
General Stability | Organism |
---|---|
glucose dehydrogenase isoenzymes are stabilized in presence of 2 M NaCl. The effect is especially large for GlcDH-III, which is the most unstable enzyme | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
NADP+ | pH 8.0, 30°C, isoenzyme GlcDH II | Priestia megaterium | |
0.02 | - |
NADP+ | pH 8.0, 30°C, isoenzyme GlcDH I | Priestia megaterium | |
0.081 | - |
NAD+ | pH 8.0, 30°C, isoenzyme GlcDH I | Priestia megaterium | |
0.088 | - |
NAD+ | pH 8.0, 30°C, isoenzyme GlcDH IV | Priestia megaterium | |
0.16 | - |
NAD+ | pH 8.0, 30°C, isoenzyme GlcDH III | Priestia megaterium | |
0.99 | - |
NAD+ | pH 8.0, 30°C, isoenzyme GlcDH II | Priestia megaterium | |
1.9 | - |
NADP+ | pH 8.0, 30°C, isoenzyme GlcDH IV | Priestia megaterium | |
4.5 | - |
beta-D-glucose | pH 8.0, 30°C, isoenzyme GlcDH IV, coenzyme NADP+ | Priestia megaterium | |
4.6 | - |
beta-D-glucose | pH 8.0, 30°C, isoenzyme GlcDH I, coenzyme NAD+ | Priestia megaterium | |
7.9 | - |
beta-D-glucose | pH 8.0, 30°C, isoenzyme GlcDH I, coenzyme NADP+ | Priestia megaterium | |
9.2 | - |
beta-D-glucose | pH 8.0, 30°C, isoenzyme GlcDH II, coenzyme NAD+ | Priestia megaterium | |
9.8 | - |
beta-D-glucose | pH 8.0, 30°C, isoenzyme GlcDH IV, coenzyme NAD+ | Priestia megaterium | |
12.4 | - |
beta-D-glucose | pH 8.0, 30°C, isoenzyme GlcDH II, coenzyme NADP+ | Priestia megaterium | |
18.9 | - |
NADP+ | pH 8.0, 30°C, isoenzyme GlcDH III | Priestia megaterium | |
44 | - |
beta-D-glucose | pH 8.0, 30°C, isoenzyme GlcDH III, coenzyme NAD+ | Priestia megaterium | |
135 | - |
beta-D-glucose | pH 8.0, 30°C, isoenzyme GlcDH III, coenzyme NADP+ | Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, Glc-DH-I and GlcDH-IWG3, SDS-PAGE | Priestia megaterium |
34000 | - |
x * 34000, GlcDH-II and GlcDH-IV, SDS-PAGE | Priestia megaterium |
36000 | - |
x * 36000, enzyme form GlcDH-II, SDS-PAGE | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Storage Stability | Organism |
---|---|
4°C, 50 mM sodium phosphate pH 6.5, 2 M NaCl, stable | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + NAD+ | - |
Priestia megaterium | 2-deoxy-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
2-deoxy-D-glucose + NADP+ | - |
Priestia megaterium | 2-deoxy-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
beta-D-glucose + NAD+ | although they can utilize both NAD+ and NADP+: GlcDH-III and GlcDH-IV prefer NAD+, and GlcDH-I and GlcDH-II prefer NADP+ | Priestia megaterium | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucose + NADP+ | although they can utilize both NAD+ and NADP+: GlcDH-III and GlcDH-IV prefer NAD+, and GlcDH-I and GlcDH-II prefer NADP+ | Priestia megaterium | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-galactose + NADP+ | - |
Priestia megaterium | D-galactono-1,5-lactone + NADPH | - |
? | |
D-glucosamine + NAD+ | - |
Priestia megaterium | D-glucosamino-1,5-lactone + NADH | - |
? | |
D-glucosamine + NADP+ | - |
Priestia megaterium | D-glucosamino-1,5-lactone + NADPH | - |
? | |
D-mannose + NAD+ | - |
Priestia megaterium | D-mannono-1,5-lactone + NADH | - |
? | |
D-mannose + NADP+ | - |
Priestia megaterium | D-mannono-1,5-lactone + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, Glc-DH-I and GlcDH-IWG3, SDS-PAGE | Priestia megaterium |
? | x * 34000, GlcDH-II and GlcDH-IV, SDS-PAGE | Priestia megaterium |
? | x * 36000, enzyme form GlcDH-II, SDS-PAGE | Priestia megaterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
20 min, isoenzyme GlcDH-III, without NaCl, about 55% loss of activity | Priestia megaterium |
45 | - |
20 min, isoenzyme GlcDH-IV, without NaCl, about 35% loss of activity, isoenzyme IWG3, without NaCl, about 10% loss of activity | Priestia megaterium |
50 | - |
20 min, isoenzyme GlcDH-IWG3, without NaCl, complete loss of activity, isoenzymes GlcDH-II and GlcDH-IWG3 are stable in presence of 2 M NaCl | Priestia megaterium |
65 | - |
20 min, in presence of 2 M NaCl, isoenzyme GlcDH-III loses about 30% of initial activity, isoenzyme GlcDH-IV loses about 10% of initial activity, isoenzyme IWG2 is stable | Priestia megaterium |
70 | - |
20 min, presence of 2 mM NaCl, complete inactivation of isoenzyme GlcDH-III and GlcDH-IV, isoenzyme GlcDH-IWG3 loses 30% of initial activity | Priestia megaterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
120 | - |
beta-D-glucose | pH 8.0, 30°C, coenzyme NADP+, isoenzyme GlcDH I | Priestia megaterium | |
140 | - |
beta-D-glucose | pH 8.0, 30°C, coenzyme NADP+, isoenzyme GlcDH II | Priestia megaterium | |
190 | - |
beta-D-glucose | pH 8.0, 30°C, coenzyme NAD+, isoenzymes GlcDH I and GlcDH II | Priestia megaterium | |
420 | - |
beta-D-glucose | pH 8.0, 30°C, coenzyme NAD+, isoenzyme GlcDH IV | Priestia megaterium | |
430 | - |
beta-D-glucose | pH 8.0, 30°C, coenzyme NAD+, isoenzyme GlcDH III | Priestia megaterium | |
462 | - |
beta-D-glucose | pH 8.0, 30°C, coenzyme NADP, isoenzyme GlcDH IV | Priestia megaterium | |
802 | - |
beta-D-glucose | pH 8.0, 30°C, coenzyme NADP+, isoenzyme GlcDH III | Priestia megaterium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
isoenzyme GlcDHIII has no stable pH-region without NaCl | Priestia megaterium |
5 | 10 | 20 min, in presence of 2 M NaCl, isoenzyme GlcDH-III is stable | Priestia megaterium |
5.5 | 10 | 20 min, in presence of 2 M NaCl, isoenzyme GlcDH-V is stable | Priestia megaterium |
6 | 9 | 20 min, in presence of 2 M NaCl, isoenzyme GlcDH-IWG3 is stable | Priestia megaterium |
6.5 | 7 | 20 min, without NaCl, isoenzyme GlcDH-IWG3 and GlcDH-III are stable | Priestia megaterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | although they can utilize both NAD+ and NADP+: GlcDH-III and GlcDH-IV prefer NAD+, and GlcDH-I and GlcDH-II prefer NADP+ | Priestia megaterium | |
NADP+ | although they can utilize both NAD+ and NADP+: GlcDH-III and GlcDH-IV prefer NAD+, and GlcDH-I and GlcDH-II prefer NADP+ | Priestia megaterium |