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Literature summary for 1.1.1.47 extracted from

  • Nagao, T.; Mitamura, T.; Wang, X.H.; Negoro, S.; Yomo, T.; Urabe, I.; Okada, H.
    Cloning, nucleotide sequences, and enzymatic properties of glucose dehydrogenase isoenzymes from Bacillus megaterium IAM1030 (1992), J. Bacteriol., 174, 5013-5020.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, isoenzymes III and IV Priestia megaterium

General Stability

General Stability Organism
glucose dehydrogenase isoenzymes are stabilized in presence of 2 M NaCl. The effect is especially large for GlcDH-III, which is the most unstable enzyme Priestia megaterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
NADP+ pH 8.0, 30°C, isoenzyme GlcDH II Priestia megaterium
0.02
-
NADP+ pH 8.0, 30°C, isoenzyme GlcDH I Priestia megaterium
0.081
-
NAD+ pH 8.0, 30°C, isoenzyme GlcDH I Priestia megaterium
0.088
-
NAD+ pH 8.0, 30°C, isoenzyme GlcDH IV Priestia megaterium
0.16
-
NAD+ pH 8.0, 30°C, isoenzyme GlcDH III Priestia megaterium
0.99
-
NAD+ pH 8.0, 30°C, isoenzyme GlcDH II Priestia megaterium
1.9
-
NADP+ pH 8.0, 30°C, isoenzyme GlcDH IV Priestia megaterium
4.5
-
beta-D-glucose pH 8.0, 30°C, isoenzyme GlcDH IV, coenzyme NADP+ Priestia megaterium
4.6
-
beta-D-glucose pH 8.0, 30°C, isoenzyme GlcDH I, coenzyme NAD+ Priestia megaterium
7.9
-
beta-D-glucose pH 8.0, 30°C, isoenzyme GlcDH I, coenzyme NADP+ Priestia megaterium
9.2
-
beta-D-glucose pH 8.0, 30°C, isoenzyme GlcDH II, coenzyme NAD+ Priestia megaterium
9.8
-
beta-D-glucose pH 8.0, 30°C, isoenzyme GlcDH IV, coenzyme NAD+ Priestia megaterium
12.4
-
beta-D-glucose pH 8.0, 30°C, isoenzyme GlcDH II, coenzyme NADP+ Priestia megaterium
18.9
-
NADP+ pH 8.0, 30°C, isoenzyme GlcDH III Priestia megaterium
44
-
beta-D-glucose pH 8.0, 30°C, isoenzyme GlcDH III, coenzyme NAD+ Priestia megaterium
135
-
beta-D-glucose pH 8.0, 30°C, isoenzyme GlcDH III, coenzyme NADP+ Priestia megaterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
x * 29000, Glc-DH-I and GlcDH-IWG3, SDS-PAGE Priestia megaterium
34000
-
x * 34000, GlcDH-II and GlcDH-IV, SDS-PAGE Priestia megaterium
36000
-
x * 36000, enzyme form GlcDH-II, SDS-PAGE Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-

Storage Stability

Storage Stability Organism
4°C, 50 mM sodium phosphate pH 6.5, 2 M NaCl, stable Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + NAD+
-
Priestia megaterium 2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
?
2-deoxy-D-glucose + NADP+
-
Priestia megaterium 2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
?
beta-D-glucose + NAD+ although they can utilize both NAD+ and NADP+: GlcDH-III and GlcDH-IV prefer NAD+, and GlcDH-I and GlcDH-II prefer NADP+ Priestia megaterium D-glucono-1,5-lactone + NADH + H+
-
?
beta-D-glucose + NADP+ although they can utilize both NAD+ and NADP+: GlcDH-III and GlcDH-IV prefer NAD+, and GlcDH-I and GlcDH-II prefer NADP+ Priestia megaterium D-glucono-1,5-lactone + NADPH + H+
-
?
D-galactose + NADP+
-
Priestia megaterium D-galactono-1,5-lactone + NADPH
-
?
D-glucosamine + NAD+
-
Priestia megaterium D-glucosamino-1,5-lactone + NADH
-
?
D-glucosamine + NADP+
-
Priestia megaterium D-glucosamino-1,5-lactone + NADPH
-
?
D-mannose + NAD+
-
Priestia megaterium D-mannono-1,5-lactone + NADH
-
?
D-mannose + NADP+
-
Priestia megaterium D-mannono-1,5-lactone + NADPH
-
?

Subunits

Subunits Comment Organism
? x * 29000, Glc-DH-I and GlcDH-IWG3, SDS-PAGE Priestia megaterium
? x * 34000, GlcDH-II and GlcDH-IV, SDS-PAGE Priestia megaterium
? x * 36000, enzyme form GlcDH-II, SDS-PAGE Priestia megaterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
20 min, isoenzyme GlcDH-III, without NaCl, about 55% loss of activity Priestia megaterium
45
-
20 min, isoenzyme GlcDH-IV, without NaCl, about 35% loss of activity, isoenzyme IWG3, without NaCl, about 10% loss of activity Priestia megaterium
50
-
20 min, isoenzyme GlcDH-IWG3, without NaCl, complete loss of activity, isoenzymes GlcDH-II and GlcDH-IWG3 are stable in presence of 2 M NaCl Priestia megaterium
65
-
20 min, in presence of 2 M NaCl, isoenzyme GlcDH-III loses about 30% of initial activity, isoenzyme GlcDH-IV loses about 10% of initial activity, isoenzyme IWG2 is stable Priestia megaterium
70
-
20 min, presence of 2 mM NaCl, complete inactivation of isoenzyme GlcDH-III and GlcDH-IV, isoenzyme GlcDH-IWG3 loses 30% of initial activity Priestia megaterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
120
-
beta-D-glucose pH 8.0, 30°C, coenzyme NADP+, isoenzyme GlcDH I Priestia megaterium
140
-
beta-D-glucose pH 8.0, 30°C, coenzyme NADP+, isoenzyme GlcDH II Priestia megaterium
190
-
beta-D-glucose pH 8.0, 30°C, coenzyme NAD+, isoenzymes GlcDH I and GlcDH II Priestia megaterium
420
-
beta-D-glucose pH 8.0, 30°C, coenzyme NAD+, isoenzyme GlcDH IV Priestia megaterium
430
-
beta-D-glucose pH 8.0, 30°C, coenzyme NAD+, isoenzyme GlcDH III Priestia megaterium
462
-
beta-D-glucose pH 8.0, 30°C, coenzyme NADP, isoenzyme GlcDH IV Priestia megaterium
802
-
beta-D-glucose pH 8.0, 30°C, coenzyme NADP+, isoenzyme GlcDH III Priestia megaterium

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
isoenzyme GlcDHIII has no stable pH-region without NaCl Priestia megaterium
5 10 20 min, in presence of 2 M NaCl, isoenzyme GlcDH-III is stable Priestia megaterium
5.5 10 20 min, in presence of 2 M NaCl, isoenzyme GlcDH-V is stable Priestia megaterium
6 9 20 min, in presence of 2 M NaCl, isoenzyme GlcDH-IWG3 is stable Priestia megaterium
6.5 7 20 min, without NaCl, isoenzyme GlcDH-IWG3 and GlcDH-III are stable Priestia megaterium

Cofactor

Cofactor Comment Organism Structure
NAD+ although they can utilize both NAD+ and NADP+: GlcDH-III and GlcDH-IV prefer NAD+, and GlcDH-I and GlcDH-II prefer NADP+ Priestia megaterium
NADP+ although they can utilize both NAD+ and NADP+: GlcDH-III and GlcDH-IV prefer NAD+, and GlcDH-I and GlcDH-II prefer NADP+ Priestia megaterium