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Literature summary for 1.1.1.47 extracted from

  • Siebers, B.; Wendisch, V.F.; Hensel, R.
    Carbohydrate metabolism in thermoproteus tenax: in vivo utilization of the non-phosphorylative Entner-Doudoroff pathway and characterization of its first enzyme, glucose dehydrogenase (1997), Arch. Microbiol., 168, 120-127.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NADP+ 2 mM NADP+ transforms enzyme at 70°C within 10 min from inactive to active state Thermoproteus tenax

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
beta-D-glucose pH 7.0, 70°C Thermoproteus tenax
0.7
-
NADP+ pH 7.0, 70°C Thermoproteus tenax
0.86
-
NAD+ pH 7.0, 70°C Thermoproteus tenax
8
-
xylose pH 7.0, 70°C Thermoproteus tenax

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
2 * 41000, SDS-PAGE Thermoproteus tenax
84000
-
in the presence of 1 mM NADP+, without NADP+ enzyme forms aggregates, gel filtration Thermoproteus tenax

Organism

Organism UniProt Comment Textmining
Thermoproteus tenax
-
-
-

Purification (Commentary)

Purification (Comment) Organism
80°C, anion-exchange chromatography, hydrophobic chromatography, gel filtration with 1 mM NADP+ Thermoproteus tenax

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
202
-
-
Thermoproteus tenax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + NAD+
-
Thermoproteus tenax D-glucono-1,5-lactone + NADH + H+
-
ir
beta-D-glucose + NADP+ 100fold higher affinity for NADP+ than for NAD+ Thermoproteus tenax D-glucono-1,5-lactone + NADPH + H+
-
ir
D-xylose + NADP+
-
Thermoproteus tenax D-xylono-1,5-lactone + NADPH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 41000, SDS-PAGE Thermoproteus tenax

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
103
-
half-life: 10 min Thermoproteus tenax

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Thermoproteus tenax
NADP+
-
Thermoproteus tenax