Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NADP+ | 2 mM NADP+ transforms enzyme at 70°C within 10 min from inactive to active state | Thermoproteus tenax |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
beta-D-glucose | pH 7.0, 70°C | Thermoproteus tenax | |
0.7 | - |
NADP+ | pH 7.0, 70°C | Thermoproteus tenax | |
0.86 | - |
NAD+ | pH 7.0, 70°C | Thermoproteus tenax | |
8 | - |
xylose | pH 7.0, 70°C | Thermoproteus tenax |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
2 * 41000, SDS-PAGE | Thermoproteus tenax |
84000 | - |
in the presence of 1 mM NADP+, without NADP+ enzyme forms aggregates, gel filtration | Thermoproteus tenax |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoproteus tenax | - |
- |
- |
Purification (Comment) | Organism |
---|---|
80°C, anion-exchange chromatography, hydrophobic chromatography, gel filtration with 1 mM NADP+ | Thermoproteus tenax |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
202 | - |
- |
Thermoproteus tenax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + NAD+ | - |
Thermoproteus tenax | D-glucono-1,5-lactone + NADH + H+ | - |
ir | |
beta-D-glucose + NADP+ | 100fold higher affinity for NADP+ than for NAD+ | Thermoproteus tenax | D-glucono-1,5-lactone + NADPH + H+ | - |
ir | |
D-xylose + NADP+ | - |
Thermoproteus tenax | D-xylono-1,5-lactone + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41000, SDS-PAGE | Thermoproteus tenax |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
103 | - |
half-life: 10 min | Thermoproteus tenax |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermoproteus tenax | |
NADP+ | - |
Thermoproteus tenax |