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Literature summary for 1.1.1.46 extracted from

  • Johnsen, U.; Sutter, J.M.; Zaiß, H.; Schönheit, P.
    L-Arabinose degradation pathway in the haloarchaeon Haloferax volcanii involves a novel type of L-arabinose dehydrogenase (2013), Extremophiles, 17, 897-909.
    View publication on PubMed
Search for more literature for 1.1.1.46

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3)-pLysS cells Haloferax volcanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 NAD+ at pH 8.5 and 45°C Haloferax volcanii
2.1
-
 NADP+ at pH 8.5 and 45°C Haloferax volcanii

Metals/Ions

Metals/Ions Comment Organism Structure
K+ dependent on with with maximal activity at 1.5 M Haloferax volcanii
Na+ dependent on with with maximal activity at 1.0 M Haloferax volcanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27800
-
-
 Haloferax volcanii
28000
-
 2 * 28000, SDS-PAGE Haloferax volcanii
130000
-
 gel filtration Haloferax volcanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arabinose + NAD+ Haloferax volcanii
-
 L-arabinono-1,4-lactone + NADH + H+
-
 ?
L-arabinose + NAD+ Haloferax volcanii H26
-
 L-arabinono-1,4-lactone + NADH + H+
-
 ?
L-arabinose + NAD+ Haloferax volcanii DSM 3757
-
 L-arabinono-1,4-lactone + NADH + H+
-
 ?
L-arabinose + NADP+ Haloferax volcanii
-
 L-arabinono-1,4-lactone + NADPH + H+
-
 ?
L-arabinose + NADP+ Haloferax volcanii H26
-
 L-arabinono-1,4-lactone + NADPH + H+
-
 ?
L-arabinose + NADP+ Haloferax volcanii DSM 3757
-
 L-arabinono-1,4-lactone + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Haloferax volcanii D4GP33
-
-
Haloferax volcanii H26 D4GP33
-
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose CL 4B column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration Haloferax volcanii

Renatured (Commentary)

Renatured (Comment) Organism
refolding buffer contains 3 M KCl, L-arabinose, NADP+ and glutathione Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arabinose + NAD+
-
 Haloferax volcanii L-arabinono-1,4-lactone + NADH + H+
-
 ?
L-arabinose + NAD+
-
 Haloferax volcanii H26 L-arabinono-1,4-lactone + NADH + H+
-
 ?
L-arabinose + NAD+
-
 Haloferax volcanii DSM 3757 L-arabinono-1,4-lactone + NADH + H+
-
 ?
L-arabinose + NADP+
-
 Haloferax volcanii L-arabinono-1,4-lactone + NADPH + H+
-
 ?
L-arabinose + NADP+
-
 Haloferax volcanii H26 L-arabinono-1,4-lactone + NADPH + H+
-
 ?
L-arabinose + NADP+
-
 Haloferax volcanii DSM 3757 L-arabinono-1,4-lactone + NADPH + H+
-
 ?
additional information no activity with ribose, D-glucose, D-talose, D-galactose, D-arabinose, D-xylose, D-mannose, L-mannose and D-fructose Haloferax volcanii ?
-
 ?
additional information no activity with ribose, D-glucose, D-talose, D-galactose, D-arabinose, D-xylose, D-mannose, L-mannose and D-fructose Haloferax volcanii H26 ?
-
 ?
additional information no activity with ribose, D-glucose, D-talose, D-galactose, D-arabinose, D-xylose, D-mannose, L-mannose and D-fructose Haloferax volcanii DSM 3757 ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 2 * 28000, SDS-PAGE Haloferax volcanii
homotetramer 2 * 27800, calculated from amino acid sequence Haloferax volcanii

Synonyms

Synonyms Comment Organism
L-arabinose dehydrogenase
-
 Haloferax volcanii
L-AraDH
-
 Haloferax volcanii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Haloferax volcanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Haloferax volcanii

pH Range

pH Minimum pH Maximum Comment Organism
6 10 50% activity at pH 6.0 and 10.0 Haloferax volcanii

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme shows slight preference for NADP+ over NAD+ Haloferax volcanii
NADP+ the enzyme shows slight preference for NADP+ over NAD+ Haloferax volcanii

General Information

General Information Comment Organism
metabolism the enzyme is functionally involved in L-arabinose catabolism but not in D-xylose degradation Haloferax volcanii