Application | Comment | Organism |
---|---|---|
synthesis | NADPH regeneration. When coupled with glucose-6-phosphate dehydrogenase the enzyme generates two moles of NADPH per mole of glucose-6-phosphate | Thermotoga maritima |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADP+ | 80°C, pH 7.5 | Thermotoga maritima | |
0.011 | - |
6-phospho-D-gluconate | 80°C, pH 7.5 | Thermotoga maritima | |
0.38 | - |
NAD+ | 80°C, pH 7.5 | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9WYR9 | - |
- |
Thermotoga maritima DSM 3109 | Q9WYR9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-phospho-D-gluconate + NAD+ | the enzyme prefers NADP+ to NAD+ as an electron acceptor. Km-value for NAD+ is 38fold higher than the Km-value for NADP+ | Thermotoga maritima | D-ribulose 5-phosphate + CO2 + NADH + H+ | - |
? | |
6-phospho-D-gluconate + NAD+ | the enzyme prefers NADP+ to NAD+ as an electron acceptor. Km-value for NAD+ is 38fold higher than the Km-value for NADP+ | Thermotoga maritima DSM 3109 | D-ribulose 5-phosphate + CO2 + NADH + H+ | - |
? | |
6-phospho-D-gluconate + NADP+ | the enzyme preferrs NADP+ to NAD+ as an electron acceptor | Thermotoga maritima | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
? | |
6-phospho-D-gluconate + NADP+ | the enzyme preferrs NADP+ to NAD+ as an electron acceptor | Thermotoga maritima DSM 3109 | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6PGDH | - |
Thermotoga maritima |
TM0438 | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 95 | 70°C: about 55% of maximal activity, 95°C: about 65% of maximal activity | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life: 140 h | Thermotoga maritima |
90 | - |
half-life: 48 h | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
325 | - |
NADP+ | 80°C, pH 7.5 | Thermotoga maritima | |
325 | - |
6-phospho-D-gluconate | 80°C, pH 7.5 | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | about 70% of the activity remains at pH 6.0 and 9.0 | Thermotoga maritima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | the enzyme preferrs NADP+ to NAD+ as an electron acceptor | Thermotoga maritima |
Organism | Comment | Expression |
---|---|---|
Thermotoga maritima | through numerous approaches including expression vectors, hosts, cultivation conditions, inducers, and codon-optimization of the 6pgdh gene, the soluble 6PGDH expression levels are enhanced to about 250 mg per liter of culture by more than 500fold | up |