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Literature summary for 1.1.1.44 extracted from

  • Huang, R.; Chen, H.; Zhong, C.; Kim, J.E.; Zhang, Y.H.
    High-throughput screening of coenzyme preference change of thermophilic 6-phosphogluconate dehydrogenase from NADP+ to NAD+ (2016), Sci. Rep., 6, 32644.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Moorella thermoacetica

Protein Variants

Protein Variants Comment Organism
A30C/R31I/T32K the mutant exhibits a 118.5fold reversal of coenzyme selectivity from NADP+ to NAD+ Moorella thermoacetica
A30D/R31I/T32I the mutant exhibits a 4278fold reversal of coenzyme selectivity from NADP+ to NAD+ Moorella thermoacetica
A30E/R31I/T32D the mutant exhibits a 1293fold reversal of coenzyme selectivity from NADP+ to NAD+ Moorella thermoacetica
R31I the mutant exhibits a 6.9fold reversal of coenzyme selectivity from NADP+ to NAD+ Moorella thermoacetica
R31I/T32G the mutant exhibits a 22.1fold reversal of coenzyme selectivity from NADP+ to NAD+ Moorella thermoacetica
R31T the mutant exhibits a 3.5fold reversal of coenzyme selectivity from NADP+ to NAD+ Moorella thermoacetica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01187
-
NAD+ mutant enzyme A30D/R31I/T32I, at pH 7.5 and 50°C Moorella thermoacetica
0.0139
-
NADP+ wild type enzyme, at pH 7.5 and 50°C Moorella thermoacetica
0.0265
-
NADP+ mutant enzyme R31I, at pH 7.5 and 50°C Moorella thermoacetica
0.0355
-
NADP+ mutant enzyme R31T, at pH 7.5 and 50°C Moorella thermoacetica
0.1044
-
NADP+ mutant enzyme R31I/T32G, at pH 7.5 and 50°C Moorella thermoacetica
0.127
-
NAD+ mutant enzyme A30E/R31I/T32D, at pH 7.5 and 50°C Moorella thermoacetica
0.228
-
NADP+ mutant enzyme A30D/R31I/T32I, at pH 7.5 and 50°C Moorella thermoacetica
0.354
-
NAD+ mutant enzyme R31I, at pH 7.5 and 50°C Moorella thermoacetica
0.362
-
NAD+ mutant enzyme R31I/T32G, at pH 7.5 and 50°C Moorella thermoacetica
0.404
-
NAD+ mutant enzyme A30C/R31I/T32K, at pH 7.5 and 50°C Moorella thermoacetica
0.605
-
NAD+ mutant enzyme R31T, at pH 7.5 and 50°C Moorella thermoacetica
0.66
-
NADP+ mutant enzyme A30E/R31I/T32D, at pH 7.5 and 50°C Moorella thermoacetica
0.698
-
NADP+ mutant enzyme A30C/R31I/T32K, at pH 7.5 and 50°C Moorella thermoacetica
1.397
-
NAD+ wild type enzyme, at pH 7.5 and 50°C Moorella thermoacetica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-phospho-D-gluconate + NADP+ Moorella thermoacetica
-
D-ribulose 5-phosphate + CO2 + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica A0A1J5NX54
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NAD+ very low activity with NAD+ Moorella thermoacetica D-ribulose 5-phosphate + CO2 + NADH + H+
-
?
6-phospho-D-gluconate + NADP+
-
Moorella thermoacetica D-ribulose 5-phosphate + CO2 + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
6-phosphogluconate dehydrogenase
-
Moorella thermoacetica
6PGDH
-
Moorella thermoacetica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.81
-
NADP+ mutant enzyme A30D/R31I/T32I, at pH 7.5 and 50°C Moorella thermoacetica
3.1
-
NADP+ mutant enzyme A30E/R31I/T32D, at pH 7.5 and 50°C Moorella thermoacetica
5.75
-
NAD+ mutant enzyme A30D/R31I/T32I, at pH 7.5 and 50°C Moorella thermoacetica
6
-
NAD+ mutant enzyme A30C/R31I/T32K, at pH 7.5 and 50°C Moorella thermoacetica
6.23
-
NADP+ mutant enzyme A30C/R31I/T32K, at pH 7.5 and 50°C Moorella thermoacetica
8.73
-
NADP+ wild type enzyme, at pH 7.5 and 50°C Moorella thermoacetica
9.12
-
NAD+ mutant enzyme R31T, at pH 7.5 and 50°C Moorella thermoacetica
10.8
-
NAD+ mutant enzyme A30E/R31I/T32D, at pH 7.5 and 50°C Moorella thermoacetica
10.83
-
NADP+ mutant enzyme R31T, at pH 7.5 and 50°C Moorella thermoacetica
11.53
-
NADP+ mutant enzyme R31I, at pH 7.5 and 50°C Moorella thermoacetica
11.9
-
NADP+ mutant enzyme R31I/T32G, at pH 7.5 and 50°C Moorella thermoacetica
12.6
-
NAD+ wild type enzyme, at pH 7.5 and 50°C Moorella thermoacetica
12.94
-
NAD+ mutant enzyme R31I/T32G, at pH 7.5 and 50°C Moorella thermoacetica
15.01
-
NAD+ mutant enzyme R31I, at pH 7.5 and 50°C Moorella thermoacetica

Cofactor

Cofactor Comment Organism Structure
NAD+ the catalytic efficiency for NADP+ is far higher than that for NAD+ Moorella thermoacetica
NADP+ the catalytic efficiency for NADP+ is far higher than that for NAD+ Moorella thermoacetica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.7
-
NADP+ mutant enzyme A30E/R31I/T32D, at pH 7.5 and 50°C Moorella thermoacetica
7.9
-
NADP+ mutant enzyme A30D/R31I/T32I, at pH 7.5 and 50°C Moorella thermoacetica
8.9
-
NADP+ mutant enzyme A30C/R31I/T32K, at pH 7.5 and 50°C Moorella thermoacetica
9
-
NAD+ wild type enzyme, at pH 7.5 and 50°C Moorella thermoacetica
14.8
-
NAD+ mutant enzyme A30C/R31I/T32K, at pH 7.5 and 50°C Moorella thermoacetica
15
-
NAD+ mutant enzyme R31T, at pH 7.5 and 50°C Moorella thermoacetica
35.8
-
NAD+ mutant enzyme R31I/T32G, at pH 7.5 and 50°C Moorella thermoacetica
42.4
-
NAD+ mutant enzyme R31I, at pH 7.5 and 50°C Moorella thermoacetica
85.1
-
NAD+ mutant enzyme A30E/R31I/T32D, at pH 7.5 and 50°C Moorella thermoacetica
114
-
NADP+ mutant enzyme R31I/T32G, at pH 7.5 and 50°C Moorella thermoacetica
305.6
-
NADP+ mutant enzyme R31T, at pH 7.5 and 50°C Moorella thermoacetica
435
-
NADP+ mutant enzyme R31I, at pH 7.5 and 50°C Moorella thermoacetica
484.2
-
NAD+ mutant enzyme A30D/R31I/T32I, at pH 7.5 and 50°C Moorella thermoacetica
628.8
-
NADP+ wild type enzyme, at pH 7.5 and 50°C Moorella thermoacetica