Literature summary for 1.1.1.44 extracted from

Sun, L.D.; Luo, Z.; Hu, W.; Zhuo, M.Q.; Zheng, J.L.; Chen, Q.L.; Liang, X.F.; Xiong, B.X.
Purification and characterization of 6-phosphogluconate dehydrogenase (6-PGD) from grass carp (Ctenopharyngodon idella) hepatopancreas (2013), Indian J. Biochem. Biophys., 50, 554-561.

Search for more literature for 1.1.1.44

Inhibitors

Inhibitors	Comment	Organism	Structure
6-phospho-D-gluconate	substrate inhibition	Ctenopharyngodon idella
Al3+	-	Ctenopharyngodon idella
Cd2+	competitive inhibition	Ctenopharyngodon idella
Cu2+	competitive inhibition	Ctenopharyngodon idella
NADP+	substrate inhibition	Ctenopharyngodon idella
NADPH	competitive inhibition	Ctenopharyngodon idella
Zn2+	competitive inhibition	Ctenopharyngodon idella

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0052	-	NADP+	at pH 8.0 and 25°C	Ctenopharyngodon idella
0.019	-	6-phospho-D-gluconate	at pH 8.0 and 25°C	Ctenopharyngodon idella

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
57000	-	2 * 57000, SDS-PAGE	Ctenopharyngodon idella

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6-phospho-D-gluconate + NADP+	Ctenopharyngodon idella	-	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Ctenopharyngodon idella	-	grass carp	-

Purification (Commentary)

Purification (Comment)	Organism
2',5'-ADP-Sepharose 4B column chromatography	Ctenopharyngodon idella

Source Tissue

Source Tissue	Comment	Organism	Textmining
hepatopancreas	-	Ctenopharyngodon idella	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.017	-	unpurified enzyme, at pH 8.0 and 25°C	Ctenopharyngodon idella
5.259	-	after 309fold purification, at pH 8.0 and 25°C	Ctenopharyngodon idella

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6-phospho-D-gluconate + NADP+	-	Ctenopharyngodon idella	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	r

Subunits

Subunits	Comment	Organism
homodimer	2 * 57000, SDS-PAGE	Ctenopharyngodon idella

Synonyms

Synonyms	Comment	Organism
6-phosphogluconate dehydrogenase	-	Ctenopharyngodon idella
6PGD	-	Ctenopharyngodon idella

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Ctenopharyngodon idella

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Ctenopharyngodon idella

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	dependent on	Ctenopharyngodon idella

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0285	-	Cd2+	at pH 8.0 and 25°C	Ctenopharyngodon idella
0.032	-	NADPH	at pH 8.0 and 25°C	Ctenopharyngodon idella
0.084	-	Cu2+	at pH 8.0 and 25°C	Ctenopharyngodon idella
0.12	-	NADP+	at pH 8.0 and 25°C	Ctenopharyngodon idella
0.144	-	Zn2+	at pH 8.0 and 25°C	Ctenopharyngodon idella
2.05	-	6-phospho-D-gluconate	at pH 8.0 and 25°C	Ctenopharyngodon idella

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.045	-	at pH 8.0 and 25°C	Ctenopharyngodon idella	Cd2+
0.099	-	at pH 8.0 and 25°C	Ctenopharyngodon idella	Zn2+
0.293	-	at pH 8.0 and 25°C	Ctenopharyngodon idella	Cu2+
1.526	-	at pH 8.0 and 25°C	Ctenopharyngodon idella	Al3+

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Release 2023.1 (January 2023)