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Literature summary for 1.1.1.44 extracted from

  • Hanau, S.; Montin, K.; Cervellati, C.; Magnani, M.; Dallocchio, F.
    6-Phosphogluconate dehydrogenase mechanism: evidence for allosteric modulation by substrate (2010), J. Biol. Chem., 285, 21366-21371.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.44

Inhibitors

Inhibitors Comment Organism Structure
NADPH 6-phosphogluconate modulates 6PGDH activity. In the forward reaction, it suppresses inhibition by NADP and decreases inhibition by NADPH by increasing the Kd of the reduced coenzyme 6fold it increases the rate of decarboxylation of the 3-keto intermediate , whereas in the reverse reaction, it decreases the Km and removes the slow step Cyberlindnera jadinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 6-phospho-D-gluconate dimeric enzyme with two 6-phospho-D-gluconate bound, pH not specified in the publication, temperature not specified in the publication Cyberlindnera jadinii
0.07
-
 6-phospho-D-gluconate dimeric enzyme with one 6-phospho-D-gluconate bound, pH not specified in the publication, temperature not specified in the publication Cyberlindnera jadinii

Organism

Organism UniProt Comment Textmining
Cyberlindnera jadinii
-
-
-

Reaction

Reaction Comment Organism Reaction ID
6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH + H+ reductive carboxylation of ribulose-5-phosphate by 6PGDH is investigated using kinetic isotope effects. In the absence of 6-phosphogluconate, a slow step, which precedes the chemical process, is rate-limiting for the reaction, whereas in the presence of 6-phosphogluconate, the rate-limiting step follows the isotope-sensitive step. Full activity of 6PGDH is achieved when one subunit carries out the catalysis and the other subunit carries an unreacted 6-phosphogluconate (allosteric activator) Cyberlindnera jadinii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NADP+ 6-phosphogluconate modulates 6PGDH activity. In the forward reaction, it suppresses inhibition by NADP and decreases inhibition by NADPH by increasing the Kd of the reduced coenzyme 6fold it increases the rate of decarboxylation of the 3-keto intermediate , whereas in the reverse reaction, it decreases the Km and removes the slow step Cyberlindnera jadinii D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
6PGDH
-
 Cyberlindnera jadinii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24
-
 assay at Cyberlindnera jadinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
 assay at Cyberlindnera jadinii

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Cyberlindnera jadinii