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Literature summary for 1.1.1.44 extracted from

  • Wang, Y.; Zhang, Y.H.
    Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regeneration (2009), Microb. Cell Fact., 8, 30.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADP+ in 50 mM HEPES buffer (pH 7.5) containing 5 mM Mg2+, 0.5 mM Mn2+, and 0.5 mg bovine serum albumin, at 80°C Thermotoga maritima
0.011
-
6-phospho-D-gluconate in 50 mM HEPES buffer (pH 7.5) containing 5 mM Mg2+, 0.5 mM Mn2+, and 0.5 mg bovine serum albumin, at 80°C Thermotoga maritima
0.38
-
NAD+ in 50 mM HEPES buffer (pH 7.5) containing 5 mM Mg2+, 0.5 mM Mn2+, and 0.5 mg bovine serum albumin, at 80°C Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
SDS-PAGE Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NAD+ the enzyme prefers NADP+ to NAD+ as an electron acceptor Thermotoga maritima D-ribulose 5-phosphate + CO2 + NADH
-
?
6-phospho-D-gluconate + NADP+ the enzyme prefers NADP+ to NAD+ as an electron acceptor Thermotoga maritima D-ribulose 5-phosphate + CO2 + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
6-phosphogluconate dehydrogenase
-
Thermotoga maritima
6PGDH
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 95 the enzyme has an approximately 90% of its maximum activity at 80°C, and retains about 2 and about 20% of its maximum activity at 30 and 60°C, respectively Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80 90 the enzyme is highly thermostable in a 50 mM HEPES buffer (pH 6.8) containing 500 mM NaCl, 1 mM EDTA, and 5 mM beta-mercaptoethanol, it retains more than 90% activity for 48 h at 60 and 80°C and retains about 50% enzymatic activity after 48 h at 90°C, the enzyme has half-life times of 48 and 140 h at 90 and 80°C, respectively Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
6 9 about 70% of 6PGDH activities remain at pH 6.0 and 9.0 Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme prefers NADP+ to NAD+ as an electron acceptor Thermotoga maritima
NADP+ the enzyme prefers NADP+ to NAD+ as an electron acceptor Thermotoga maritima