Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADP+ | in 50 mM HEPES buffer (pH 7.5) containing 5 mM Mg2+, 0.5 mM Mn2+, and 0.5 mg bovine serum albumin, at 80°C | Thermotoga maritima | |
0.011 | - |
6-phospho-D-gluconate | in 50 mM HEPES buffer (pH 7.5) containing 5 mM Mg2+, 0.5 mM Mn2+, and 0.5 mg bovine serum albumin, at 80°C | Thermotoga maritima | |
0.38 | - |
NAD+ | in 50 mM HEPES buffer (pH 7.5) containing 5 mM Mg2+, 0.5 mM Mn2+, and 0.5 mg bovine serum albumin, at 80°C | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
SDS-PAGE | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-phospho-D-gluconate + NAD+ | the enzyme prefers NADP+ to NAD+ as an electron acceptor | Thermotoga maritima | D-ribulose 5-phosphate + CO2 + NADH | - |
? | |
6-phospho-D-gluconate + NADP+ | the enzyme prefers NADP+ to NAD+ as an electron acceptor | Thermotoga maritima | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6-phosphogluconate dehydrogenase | - |
Thermotoga maritima |
6PGDH | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 95 | the enzyme has an approximately 90% of its maximum activity at 80°C, and retains about 2 and about 20% of its maximum activity at 30 and 60°C, respectively | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 90 | the enzyme is highly thermostable in a 50 mM HEPES buffer (pH 6.8) containing 500 mM NaCl, 1 mM EDTA, and 5 mM beta-mercaptoethanol, it retains more than 90% activity for 48 h at 60 and 80°C and retains about 50% enzymatic activity after 48 h at 90°C, the enzyme has half-life times of 48 and 140 h at 90 and 80°C, respectively | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | about 70% of 6PGDH activities remain at pH 6.0 and 9.0 | Thermotoga maritima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme prefers NADP+ to NAD+ as an electron acceptor | Thermotoga maritima | |
NADP+ | the enzyme prefers NADP+ to NAD+ as an electron acceptor | Thermotoga maritima |