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Literature summary for 1.1.1.44 extracted from

  • Cervellati, C.; Li, L.; Andi, B.; Guariento, A.; Dallocchio, F.; Cook, P.F.
    Proper orientation of the nicotinamide ring of NADP is important for the precatalytic conformational change in the 6-phosphogluconate dehydrogenase reaction (2008), Biochemistry, 47, 1862-1870.
    View publication on PubMed
Search for more literature for 1.1.1.44

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15 cells Ovis aries

Protein Variants

Protein Variants Comment Organism
E131A the mutant shows a 2.3fold decrease in turnover number compared to the wild type enzyme Ovis aries
M13C the mutant shows a decrease in turnover number compared to the wild type enzyme Ovis aries
M13Q the mutant shows a 8.1fold decrease in turnover number compared to the wild type enzyme Ovis aries
M13V the mutant shows a decrease in turnover number compared to the wild type enzyme Ovis aries

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and ADP-agarose column chromatography Ovis aries

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Ovis aries
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-6-phospho-D-gluconate + NADP+
-
 Ovis aries 3-oxo-2-deoxy-6-phosphogluconate + NADPH
-
 ?
6-phospho-D-gluconate + NADP+
-
 Ovis aries D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
6-phosphogluconate dehydrogenase
-
 Ovis aries
6PGDH
-
 Ovis aries

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Ovis aries