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Literature summary for 1.1.1.44 extracted from

  • Li, L.; Dworkowski, F.S.; Cook, P.F.
    Importance in catalysis of the 6-phosphate-binding site of 6-phosphogluconate in sheep liver 6-phosphogluconate dehydrogenase (2006), J. Biol. Chem., 281, 25568-25576.
    View publication on PubMed
Search for more literature for 1.1.1.44

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain M15 Ovis aries

Protein Variants

Protein Variants Comment Organism
K260A reduced activity compared to the wild type enzyme Ovis aries
R287A reduced activity compared to the wild type enzyme Ovis aries
R446A reduced activity compared to the wild type enzyme Ovis aries
T262A activity similar to the wild type enzyme Ovis aries
Y191A reduced activity compared to the wild type enzyme Ovis aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0042
-
 NADP+ mutant enzyme T262A Ovis aries
0.005
-
 NADP+ wild type enzyme Ovis aries
0.014
-
 NADP+ mutant enzyme Y191A Ovis aries
0.016
-
 NADP+ mutant enzyme K260A Ovis aries
0.024
-
 6-phospho-D-gluconate mutant enzyme T262A Ovis aries
0.028
-
 6-phospho-D-gluconate wild type enzyme Ovis aries
0.064
-
 NADP+ mutant enzyme R446A Ovis aries
0.073
-
 NADP+ mutant enzyme R287A Ovis aries
0.15
-
 6-phospho-D-gluconate mutant enzyme K260A Ovis aries
1.04
-
 6-phospho-D-gluconate mutant enzyme Y191A Ovis aries
2 3 6-phospho-D-gluconate mutant enzyme R287A Ovis aries
3.4
-
 6-phospho-D-gluconate mutant enzyme R446A Ovis aries

Organism

Organism UniProt Comment Textmining
Ovis aries P00349
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin chromatography and ADP-agarose affinity column chromatography Ovis aries

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Ovis aries
-

Storage Stability

Storage Stability Organism
4°C, 50 mM triethanolamine and 2 mM beta-mercaptoethanol at pH 7.5, 1 year, no loss of activity Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NADP+
-
 Ovis aries D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
6-phosphogluconate dehydrogenase
-
 Ovis aries
6PGDH
-
 Ovis aries

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9.5 mutant Y191A measured by varying 6PG saturation level of NADP+ Ovis aries

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Ovis aries