Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Gold (DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A134D | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
H133Q | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
R100Q | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
R132A | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
R132C | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
R132G | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
R132H | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
R132K | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
R132N | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
R132Q | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
R132W | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
NADPH | Km less than 0.0025 mM or equal, mutant enzyme R100Q, at pH 7.5 and 21°C | Homo sapiens | |
0.005 | - |
NADPH | Km less than 0.005 mM or equal, mutant enzyme R132H, at pH 7.5 and 21°C | Homo sapiens | |
0.005 | - |
NADPH | mutant enzyme R100Q, at pH 7.5 and 37°C | Homo sapiens | |
0.01 | - |
NADPH | Km less than 0.01 mM or equal, wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
0.01 | - |
NADPH | mutant enzyme R132C, at pH 7.5 and 37°C | Homo sapiens | |
0.015 | - |
isocitrate | wild type enzyme, at pH 7.5 and 21°C | Homo sapiens | |
0.025 | - |
NADPH | Km less than 0.025 mM or equal, mutant enzyme R132C, at pH 7.5 and 21°C | Homo sapiens | |
0.025 | - |
NADPH | Km less than 0.025 mM or equal, mutant enzyme R132G, at pH 7.5 and 21°C | Homo sapiens | |
0.025 | - |
NADPH | Km less than 0.025 mM or equal, mutant enzyme R132H, at pH 7.5 and 37°C | Homo sapiens | |
0.025 | - |
NADPH | Km less than 0.025 mM, mutant enzyme R132G, at pH 7.5 and 37°C | Homo sapiens | |
0.03 | - |
NADP+ | wild type enzyme, at pH 7.5 and 21°C | Homo sapiens | |
0.067 | - |
NADP+ | mutant enzyme R132G, at pH 7.5 and 37°C | Homo sapiens | |
0.07 | - |
NADP+ | mutant enzyme R100Q, at pH 7.5 and 21°C | Homo sapiens | |
0.08 | - |
NADP+ | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
0.101 | - |
NADP+ | mutant enzyme H133Q, at pH 7.5 and 21°C | Homo sapiens | |
0.11 | - |
2-oxoglutarate | mutant enzyme R132A, at pH 7.5 and 37°C | Homo sapiens | |
0.14 | - |
NADP+ | mutant enzyme R132G, at pH 7.5 and 21°C | Homo sapiens | |
0.16 | - |
NADP+ | mutant enzyme H133Q, at pH 7.5 and 37°C | Homo sapiens | |
0.18 | - |
NADP+ | mutant enzyme R100Q, at pH 7.5 and 37°C | Homo sapiens | |
0.22 | - |
isocitrate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
0.26 | - |
2-oxoglutarate | mutant enzyme R132Q, at pH 7.5 and 37°C | Homo sapiens | |
0.28 | - |
isocitrate | mutant enzyme H133Q, at pH 7.5 and 21°C | Homo sapiens | |
0.3 | - |
2-oxoglutarate | mutant enzyme R132G, at pH 7.5 and 21°C | Homo sapiens | |
0.34 | - |
2-oxoglutarate | mutant enzyme R132G, at pH 7.5 and 37°C | Homo sapiens | |
0.36 | - |
2-oxoglutarate | mutant enzyme R132C, at pH 7.5 and 21°C | Homo sapiens | |
0.36 | - |
2-oxoglutarate | mutant enzyme R132C, at pH 7.5 and 37°C | Homo sapiens | |
0.4 | - |
isocitrate | mutant enzyme H133Q, at pH 7.5 and 37°C | Homo sapiens | |
0.5 | - |
2-oxoglutarate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
0.58 | - |
NADP+ | mutant enzyme R132C, at pH 7.5 and 21°C | Homo sapiens | |
0.61 | - |
2-oxoglutarate | mutant enzyme R132K, at pH 7.5 and 37°C | Homo sapiens | |
0.7 | - |
NADP+ | mutant enzyme A134D, at pH 7.5 and 21°C | Homo sapiens | |
0.75 | - |
NADP+ | mutant enzyme R132C, at pH 7.5 and 37°C | Homo sapiens | |
0.8 | - |
isocitrate | mutant enzyme R132Q, at pH 7.5 and 37°C | Homo sapiens | |
0.82 | - |
2-oxoglutarate | mutant enzyme R132W, at pH 7.5 and 37°C | Homo sapiens | |
1 | - |
NADP+ | mutant enzyme R132H, at pH 7.5 and 21°C | Homo sapiens | |
1.1 | - |
2-oxoglutarate | mutant enzyme R132H, at pH 7.5 and 37°C | Homo sapiens | |
1.1 | - |
isocitrate | mutant enzyme R132K, at pH 7.5 and 37°C | Homo sapiens | |
1.2 | - |
NADP+ | mutant enzyme A134D, at pH 7.5 and 37°C | Homo sapiens | |
1.5 | - |
isocitrate | mutant enzyme R132N, at pH 7.5 and 37°C | Homo sapiens | |
1.6 | - |
NADP+ | mutant enzyme R132H, at pH 7.5 and 37°C | Homo sapiens | |
1.8 | - |
2-oxoglutarate | mutant enzyme R132H, at pH 7.5 and 21°C | Homo sapiens | |
2.7 | - |
isocitrate | mutant enzyme A134D, at pH 7.5 and 21°C | Homo sapiens | |
3.6 | - |
isocitrate | mutant enzyme R132G, at pH 7.5 and 21°C | Homo sapiens | |
3.6 | - |
isocitrate | mutant enzyme R132W, at pH 7.5 and 37°C | Homo sapiens | |
4.2 | - |
isocitrate | mutant enzyme R132H, at pH 7.5 and 37°C | Homo sapiens | |
5.3 | - |
isocitrate | mutant enzyme R132C, at pH 7.5 and 21°C | Homo sapiens | |
5.7 | - |
isocitrate | mutant enzyme R132A, at pH 7.5 and 37°C | Homo sapiens | |
6 | - |
isocitrate | mutant enzyme R132H, at pH 7.5 and 21°C | Homo sapiens | |
7 | - |
isocitrate | mutant enzyme R132G, at pH 7.5 and 37°C | Homo sapiens | |
8 | - |
isocitrate | mutant enzyme A134D, at pH 7.5 and 37°C | Homo sapiens | |
8 | - |
isocitrate | mutant enzyme R100Q, at pH 7.5 and 37°C | Homo sapiens | |
8.2 | - |
isocitrate | mutant enzyme R132C, at pH 7.5 and 37°C | Homo sapiens | |
9 | - |
isocitrate | mutant enzyme R100Q, at pH 7.5 and 21°C | Homo sapiens | |
10 | - |
2-oxoglutarate | mutant enzyme R100Q, at pH 7.5 and 21°C | Homo sapiens | |
10 | - |
2-oxoglutarate | mutant enzyme R132N, at pH 7.5 and 37°C | Homo sapiens | |
12 | - |
2-oxoglutarate | mutant enzyme R100Q, at pH 7.5 and 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
peroxisome | - |
Homo sapiens | 5777 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM used in assay conditions | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Homo sapiens | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O75874 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NADPH + H+ | the reaction is catalyzed by diverse R132 mutant enzymes | Homo sapiens | D-2-hydroxyglutarate + NADP+ | - |
r | |
isocitrate + NADP+ | - |
Homo sapiens | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
IDH1 | - |
Homo sapiens |
isocitrate dehydrogenase 1 | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
49.1 | - |
melting temperature | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
2-oxoglutarate | kcat less than 0.016 s-1 or equal, mutant enzyme H133Q, at pH 7.5 and 37°C | Homo sapiens | |
0.017 | - |
2-oxoglutarate | wild type enzyme, at pH 7.5 and 21°C | Homo sapiens | |
0.019 | - |
2-oxoglutarate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
0.019 | - |
2-oxoglutarate | kcat less than 0.019 s-1 or equal, mutant enzyme A134D, at pH 7.5 and 37°C | Homo sapiens | |
0.02 | - |
2-oxoglutarate | mutant enzyme A134D, at pH 7.5 and 21°C | Homo sapiens | |
0.034 | - |
2-oxoglutarate | mutant enzyme H133, at pH 7.5 and 21°C | Homo sapiens | |
0.047 | - |
isocitrate | mutant enzyme R132N, at pH 7.5 and 37°C | Homo sapiens | |
0.12 | - |
isocitrate | mutant enzyme R132H, at pH 7.5 and 21°C | Homo sapiens | |
0.128 | - |
2-oxoglutarate | mutant enzyme R100Q, at pH 7.5 and 21°C | Homo sapiens | |
0.2 | - |
isocitrate | mutant enzyme A134D, at pH 7.5 and 21°C | Homo sapiens | |
0.34 | - |
2-oxoglutarate | mutant enzyme R100Q, at pH 7.5 and 37°C | Homo sapiens | |
0.37 | - |
2-oxoglutarate | mutant enzyme R132A, at pH 7.5 and 37°C | Homo sapiens | |
0.43 | - |
2-oxoglutarate | mutant enzyme R132H, at pH 7.5 and 21°C | Homo sapiens | |
0.45 | - |
2-oxoglutarate | mutant enzyme R132G, at pH 7.5 and 21°C | Homo sapiens | |
0.54 | - |
2-oxoglutarate | mutant enzyme R132W, at pH 7.5 and 37°C | Homo sapiens | |
0.57 | - |
2-oxoglutarate | mutant enzyme R132K, at pH 7.5 and 37°C | Homo sapiens | |
0.79 | - |
2-oxoglutarate | mutant enzyme R132N, at pH 7.5 and 37°C | Homo sapiens | |
0.84 | - |
2-oxoglutarate | mutant enzyme R132C, at pH 7.5 and 21°C | Homo sapiens | |
1 | - |
isocitrate | mutant enzyme R132G, at pH 7.5 and 21°C | Homo sapiens | |
1.21 | - |
isocitrate | mutant enzyme R132W, at pH 7.5 and 37°C | Homo sapiens | |
1.4 | - |
isocitrate | mutant enzyme R100Q, at pH 7.5 and 21°C | Homo sapiens | |
1.59 | - |
2-oxoglutarate | mutant enzyme R132G, at pH 7.5 and 37°C | Homo sapiens | |
1.6 | - |
2-oxoglutarate | mutant enzyme R132C, at pH 7.5 and 37°C | Homo sapiens | |
1.61 | - |
isocitrate | mutant enzyme R132C, at pH 7.5 and 21°C | Homo sapiens | |
2.3 | - |
isocitrate | mutant enzyme A134D, at pH 7.5 and 37°C | Homo sapiens | |
2.4 | - |
isocitrate | mutant enzyme R132H, at pH 7.5 and 37°C | Homo sapiens | |
4.2 | - |
2-oxoglutarate | mutant enzyme R132H, at pH 7.5 and 37°C | Homo sapiens | |
4.4 | - |
isocitrate | mutant enzyme R132C, at pH 7.5 and 37°C | Homo sapiens | |
4.7 | - |
2-oxoglutarate | mutant enzyme R132Q, at pH 7.5 and 37°C | Homo sapiens | |
5.6 | - |
isocitrate | mutant enzyme R100Q, at pH 7.5 and 37°C | Homo sapiens | |
7.2 | - |
isocitrate | mutant enzyme R132K, at pH 7.5 and 37°C | Homo sapiens | |
9.2 | - |
isocitrate | mutant enzyme R132Q, at pH 7.5 and 37°C | Homo sapiens | |
9.3 | - |
isocitrate | mutant enzyme R132G, at pH 7.5 and 37°C | Homo sapiens | |
9.4 | - |
isocitrate | mutant enzyme H133Q, at pH 7.5 and 21°C | Homo sapiens | |
10.4 | - |
isocitrate | mutant enzyme R132A, at pH 7.5 and 37°C | Homo sapiens | |
11 | - |
isocitrate | wild type enzyme, at pH 7.5 and 21°C | Homo sapiens | |
45 | - |
isocitrate | mutant enzyme H133Q, at pH 7.5 and 37°C | Homo sapiens | |
85 | - |
isocitrate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
2-oxoglutarate | mutant enzyme R100Q, at pH 7.5 and 21°C | Homo sapiens | |
0.02 | - |
isocitrate | mutant enzyme R132H, at pH 7.5 and 21°C | Homo sapiens | |
0.028 | - |
2-oxoglutarate | mutant enzyme R100Q, at pH 7.5 and 37°C | Homo sapiens | |
0.031 | - |
isocitrate | mutant enzyme R132N, at pH 7.5 and 37°C | Homo sapiens | |
0.04 | - |
2-oxoglutarate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
0.074 | - |
isocitrate | mutant enzyme A134D, at pH 7.5 and 21°C | Homo sapiens | |
0.08 | - |
2-oxoglutarate | mutant enzyme R132N, at pH 7.5 and 37°C | Homo sapiens | |
0.16 | - |
isocitrate | mutant enzyme R100Q, at pH 7.5 and 21°C | Homo sapiens | |
0.24 | - |
2-oxoglutarate | mutant enzyme R132H, at pH 7.5 and 21°C | Homo sapiens | |
0.28 | - |
isocitrate | mutant enzyme R132G, at pH 7.5 and 21°C | Homo sapiens | |
0.29 | - |
isocitrate | mutant enzyme A134D, at pH 7.5 and 37°C | Homo sapiens | |
0.3 | - |
isocitrate | mutant enzyme R132C, at pH 7.5 and 21°C | Homo sapiens | |
0.34 | - |
isocitrate | mutant enzyme R132W, at pH 7.5 and 37°C | Homo sapiens | |
0.54 | - |
isocitrate | mutant enzyme R132C, at pH 7.5 and 37°C | Homo sapiens | |
0.57 | - |
isocitrate | mutant enzyme R132H, at pH 7.5 and 37°C | Homo sapiens | |
0.659 | - |
2-oxoglutarate | mutant enzyme R132W, at pH 7.5 and 37°C | Homo sapiens | |
0.7 | - |
isocitrate | mutant enzyme R100Q, at pH 7.5 and 37°C | Homo sapiens | |
0.9 | - |
2-oxoglutarate | mutant enzyme R132K, at pH 7.5 and 37°C | Homo sapiens | |
1.3 | - |
isocitrate | mutant enzyme R132G, at pH 7.5 and 37°C | Homo sapiens | |
1.5 | - |
2-oxoglutarate | mutant enzyme R132G, at pH 7.5 and 21°C | Homo sapiens | |
1.8 | - |
isocitrate | mutant enzyme R132A, at pH 7.5 and 37°C | Homo sapiens | |
2.3 | - |
2-oxoglutarate | mutant enzyme R132C, at pH 7.5 and 21°C | Homo sapiens | |
3.4 | - |
2-oxoglutarate | mutant enzyme R132A, at pH 7.5 and 37°C | Homo sapiens | |
3.8 | - |
2-oxoglutarate | mutant enzyme R132H, at pH 7.5 and 37°C | Homo sapiens | |
4.4 | - |
2-oxoglutarate | mutant enzyme R132C, at pH 7.5 and 37°C | Homo sapiens | |
5 | - |
2-oxoglutarate | mutant enzyme R132G, at pH 7.5 and 37°C | Homo sapiens | |
7 | - |
isocitrate | mutant enzyme R132K, at pH 7.5 and 37°C | Homo sapiens | |
12 | - |
isocitrate | mutant enzyme R132Q, at pH 7.5 and 37°C | Homo sapiens | |
18 | - |
2-oxoglutarate | mutant enzyme R132Q, at pH 7.5 and 37°C | Homo sapiens | |
35 | - |
isocitrate | mutant enzyme H133Q, at pH 7.5 and 21°C | Homo sapiens | |
110 | - |
isocitrate | mutant enzyme H133Q, at pH 7.5 and 37°C | Homo sapiens | |
390 | - |
isocitrate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
730 | - |
isocitrate | wild type enzyme, at pH 7.5 and 21°C | Homo sapiens |