Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Gold (DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D273L | the mutant exhibits an about 170fold decrease in catalytic efficiency, driven by a 5.4fold decrease in kcat and 31fold increase in Km as compared to the wild type enzyme | Homo sapiens |
D273L/R132H | catalytically inactive | Homo sapiens |
D273N | the mutant has a more than 500fold decrease in kcat/Km, driven primarily through more than 300fold increase in Km as compared to the wild type enzyme | Homo sapiens |
D273N/R132H | catalytically inactive | Homo sapiens |
D273S | the mutant exhibits adecrease in catalytic efficiency, driven by a 2.5fold decrease in kcat and 200fold increase in Km as compared to the wild type enzyme | Homo sapiens |
D273S/R132H | catalytically inactive | Homo sapiens |
K217M | the mutant shows reduced catalytic efficiency as compared to the wild type enzym | Homo sapiens |
K217Q | the mutant shows reduced catalytic efficiency as compared to the wild type enzym | Homo sapiens |
R132H | the mutant catalyzes the conversion of 2-oxoglutarate plus NADPH to D-2-hydroxyglutarate | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AGI-5198 | - |
Homo sapiens | |
ML309 | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 6.2 and 37°C | Homo sapiens | |
0.03 | - |
isocitrate | wild type enzyme, in Tris buffer, at pH 7.5 and 37°C | Homo sapiens | |
0.034 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 6.5 and 37°C | Homo sapiens | |
0.035 | - |
isocitrate | wild type enzyme, in Tris buffer, at pH 7.8 and 37°C | Homo sapiens | |
0.038 | - |
isocitrate | wild type enzyme, in Tris buffer, at pH 8.0 and 37°C | Homo sapiens | |
0.04 | - |
2-oxoglutarate | wild type enzyme, in Bis-Tris buffer, at pH 6.2 and 37°C | Homo sapiens | |
0.04 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 6.8 and 37°C | Homo sapiens | |
0.042 | - |
isocitrate | wild type enzyme, in Bis-Tris buffer, at pH 6.2 and 37°C | Homo sapiens | |
0.048 | - |
isocitrate | wild type enzyme, in Bis-Tris buffer, at pH 6.5 and 37°C | Homo sapiens | |
0.05 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 7.0 and 37°C | Homo sapiens | |
0.06 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 7.2 and 37°C | Homo sapiens | |
0.061 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
0.066 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 7.8 and 37°C | Homo sapiens | |
0.067 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 8.0 and 37°C | Homo sapiens | |
0.075 | - |
isocitrate | wild type enzyme, in Bis-Tris buffer, at pH 7.0 and 37°C | Homo sapiens | |
0.11 | - |
isocitrate | wild type enzyme, in Tris buffer, at pH 7.2 and 37°C | Homo sapiens | |
0.12 | - |
2-oxoglutarate | wild type enzyme, in Bis-Tris buffer, at pH 6.5 and 37°C | Homo sapiens | |
0.14 | - |
2-oxoglutarate | wild type enzyme, in Bis-Tris buffer, at pH 6.8 and 37°C | Homo sapiens | |
0.15 | - |
isocitrate | mutant enzyme K217M, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
0.246 | - |
2-oxoglutarate | wild type enzyme, in Tris buffer, at pH 7.5 and 37°C | Homo sapiens | |
0.25 | - |
isocitrate | mutant enzyme K217Q, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
0.26 | - |
2-oxoglutarate | wild type enzyme, in Bis-Tris buffer, at pH 7.0 and 37°C | Homo sapiens | |
0.26 | - |
2-oxoglutarate | wild type enzyme, in Tris buffer, at pH 7.2 and 37°C | Homo sapiens | |
0.28 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 7.2 and 37°C | Homo sapiens | |
0.46 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
0.6 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 6.2 and 37°C | Homo sapiens | |
0.65 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 6.8 and 37°C | Homo sapiens | |
0.71 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 7.0 and 37°C | Homo sapiens | |
0.87 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 7.8 and 37°C | Homo sapiens | |
1 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 6.5 and 37°C | Homo sapiens | |
1.5 | - |
2-oxoglutarate | mutant enzyme R132H, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
1.9 | - |
isocitrate | mutant enzyme D273L, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
12 | - |
isocitrate | mutant enzyme D273S, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
19 | - |
isocitrate | mutant enzyme D273N, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for catalysis, 10 mM used in assay conditions | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + CO2 + NADPH + H+ | Homo sapiens | - |
isocitrate + NADP+ | - |
r | |
isocitrate + NADP+ | Homo sapiens | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O75874 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HT-1080 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + CO2 + NADPH + H+ | - |
Homo sapiens | isocitrate + NADP+ | - |
r | |
2-oxoglutarate + NADPH + H+ | the reaction is catalyzed by mutant enzyme R132H | Homo sapiens | D-2-hydroxyglutarate + NADP+ | - |
r | |
isocitrate + NADP+ | - |
Homo sapiens | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
IDH1 | - |
Homo sapiens |
isocitrate dehydrogenase 1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.44 | - |
2-oxoglutarate | mutant enzyme R132H, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
1.6 | - |
2-oxoglutarate | wild type enzyme, in Bis-Tris buffer, at pH 6.2 and 37°C | Homo sapiens | |
1.87 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 7.8 and 37°C | Homo sapiens | |
3.2 | - |
2-oxoglutarate | wild type enzyme, in Bis-Tris buffer, at pH 6.5 and 37°C | Homo sapiens | |
3.3 | - |
2-oxoglutarate | wild type enzyme, in Bis-Tris buffer, at pH 6.8 and 37°C | Homo sapiens | |
3.4 | - |
2-oxoglutarate | wild type enzyme, in Tris buffer, at pH 7.2 and 37°C | Homo sapiens | |
3.5 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 6.2 and 37°C | Homo sapiens | |
3.6 | - |
2-oxoglutarate | wild type enzyme, in Tris buffer, at pH 7.5 and 37°C | Homo sapiens | |
3.8 | - |
2-oxoglutarate | wild type enzyme, in Bis-Tris buffer, at pH 7.0 and 37°C | Homo sapiens | |
3.8 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
3.87 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 7.2 and 37°C | Homo sapiens | |
4.1 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 6.5 and 37°C | Homo sapiens | |
4.1 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 7.0 and 37°C | Homo sapiens | |
4.9 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 6.8 and 37°C | Homo sapiens | |
7 | - |
isocitrate | mutant enzyme D273L, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
15 | - |
isocitrate | mutant enzyme D273S, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
20 | - |
isocitrate | wild type enzyme, in Bis-Tris buffer, at pH 6.2 and 37°C | Homo sapiens | |
20.2 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 6.2 and 37°C | Homo sapiens | |
22 | - |
isocitrate | mutant enzyme D273N, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
23 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 6.5 and 37°C | Homo sapiens | |
23.1 | - |
isocitrate | wild type enzyme, in Bis-Tris buffer, at pH 6.5 and 37°C | Homo sapiens | |
27.5 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 6.8 and 37°C | Homo sapiens | |
28.7 | - |
isocitrate | mutant enzyme K217Q, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
31.7 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 7.0 and 37°C | Homo sapiens | |
32 | - |
isocitrate | wild type enzyme, in Bis-Tris buffer, at pH 7.0 and 37°C | Homo sapiens | |
35.4 | - |
isocitrate | wild type enzyme, in Tris buffer, at pH 7.8 and 37°C | Homo sapiens | |
35.5 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 7.2 and 37°C | Homo sapiens | |
37.6 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
38.3 | - |
isocitrate | wild type enzyme, in Tris buffer, at pH 8.0 and 37°C | Homo sapiens | |
39 | - |
isocitrate | wild type enzyme, in Tris buffer, at pH 7.2 and 37°C | Homo sapiens | |
39.2 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 8.0 and 37°C | Homo sapiens | |
40 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 7.8 and 37°C | Homo sapiens | |
40.4 | - |
isocitrate | wild type enzyme, in Tris buffer, at pH 7.5 and 37°C | Homo sapiens | |
42 | - |
isocitrate | mutant enzyme K217M, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
2-oxoglutarate | mutant enzyme R132H, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
1.2 | - |
isocitrate | mutant enzyme D273N, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
1.3 | - |
isocitrate | mutant enzyme D273S, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
2.1 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 7.8 and 37°C | Homo sapiens | |
3.7 | - |
isocitrate | mutant enzyme D273L, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
4.1 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 6.5 and 37°C | Homo sapiens | |
5.8 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 7.0 and 37°C | Homo sapiens | |
6 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 6.2 and 37°C | Homo sapiens | |
7.5 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 6.8 and 37°C | Homo sapiens | |
8.3 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
13 | - |
2-oxoglutarate | wild type enzyme, in Tris buffer, at pH 7.2 and 37°C | Homo sapiens | |
14 | - |
2-oxoglutarate | wild type enzyme, in potassium phosphate buffer, at pH 7.2 and 37°C | Homo sapiens | |
15 | - |
2-oxoglutarate | wild type enzyme, in Tris buffer, at pH 7.5 and 37°C | Homo sapiens | |
24 | - |
2-oxoglutarate | wild type enzyme, in Bis-Tris buffer, at pH 6.8 and 37°C | Homo sapiens | |
27 | - |
2-oxoglutarate | wild type enzyme, in Bis-Tris buffer, at pH 6.5 and 37°C | Homo sapiens | |
27 | - |
2-oxoglutarate | wild type enzyme, in Bis-Tris buffer, at pH 7.0 and 37°C | Homo sapiens | |
40 | - |
2-oxoglutarate | wild type enzyme, in Bis-Tris buffer, at pH 6.2 and 37°C | Homo sapiens | |
115 | - |
isocitrate | mutant enzyme K217Q, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
280 | - |
isocitrate | mutant enzyme K217M, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
350 | - |
isocitrate | wild type enzyme, in Tris buffer, at pH 7.2 and 37°C | Homo sapiens | |
430 | - |
isocitrate | wild type enzyme, in Bis-Tris buffer, at pH 7.0 and 37°C | Homo sapiens | |
480 | - |
isocitrate | wild type enzyme, in Bis-Tris buffer, at pH 6.2 and 37°C | Homo sapiens | |
480 | - |
isocitrate | wild type enzyme, in Bis-Tris buffer, at pH 6.5 and 37°C | Homo sapiens | |
590 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 7.2 and 37°C | Homo sapiens | |
590 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 8.0 and 37°C | Homo sapiens | |
610 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 7.8 and 37°C | Homo sapiens | |
620 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 7.5 and 37°C | Homo sapiens | |
630 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 7.0 and 37°C | Homo sapiens | |
680 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 6.5 and 37°C | Homo sapiens | |
690 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 6.8 and 37°C | Homo sapiens | |
750 | - |
isocitrate | wild type enzyme, in potassium phosphate buffer, at pH 6.2 and 37°C | Homo sapiens | |
1000 | - |
isocitrate | wild type enzyme, in Tris buffer, at pH 8.0 and 37°C | Homo sapiens | |
1010 | - |
isocitrate | wild type enzyme, in Tris buffer, at pH 7.8 and 37°C | Homo sapiens | |
1400 | - |
isocitrate | wild type enzyme, in Tris buffer, at pH 7.5 and 37°C | Homo sapiens |