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Literature summary for 1.1.1.42 extracted from

  • Kim, S.Y.; Yoo, Y.H.; Park, J.W.
    Silencing of mitochondrial NADP+-dependent isocitrate dehydrogenase gene enhances glioma radiosensitivity (2013), Biochem. Biophys. Res. Commun., 433, 260-265.
    View publication on PubMed

Application

Application Comment Organism
medicine the sensitizing effect of IDPm siRNA and autophagy inhibitor on the ionizing radiation induced apoptotic cell death of glioma cells offers a novel redox-active therapeutic strategy for the treatment of cancer Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene IDPm, reverse transcription-PCR expression analysis Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information enzyme IPDM silencing by siRNA in A-172 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P48735
-
-

Source Tissue

Source Tissue Comment Organism Textmining
A-172 cell
-
Homo sapiens
-
glioma cell
-
Homo sapiens
-

Synonyms

Synonyms Comment Organism
IDPm
-
Homo sapiens
mitochondrial NADP+-dependent isocitrate dehydrogenase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

General Information

General Information Comment Organism
malfunction autophagic response to ionizing radiation in A-172 glioma cells transfected with small interfering RNA (siRNA) targeting the IDPm gene. Autophagy in A-172 transfectant cells is associated with enhanced autophagolysosome formation and GFP-LC3 punctuation/aggregation. The inhibition of autophagy by chloroquine augments apoptotic cell death of irradiated A-172 cells transfected with IDPm siRNA. The activity of cytosolic NADP+-dependent isocitrate dehydrogenase (IDPc) is not affected by the transfection of IDPm siRNA. The decreased activity caused by knockdown of IDPm siRNA is also observed in U87MG glioma cell line. When cultured A172 cells are treated with gamma-radiation, an increase in cell death is observed, but A172 cells transfected with IDPm siRNA are significantly more sensitive than control cells in this respect Homo sapiens