Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes are expressed in Escherichia coli | Caldococcus noboribetus |
Protein Variants | Comment | Organism |
---|---|---|
A325P/G326S | 50% inactivation after 10 min at 91.3°C as compared to 87.5°C for wild-type enzyme | Caldococcus noboribetus |
A336F | 50% inactivation after 10 min at 74°C as compared to 87.5°C or wild-type enzyme | Caldococcus noboribetus |
I321L | 50% inactivation after 10 min at 88.9°C as compared to 87.5°C for wild-type enzyme | Caldococcus noboribetus |
Y309I/I310L | 50% inactivation after 10 min at 88.4°C as compared to 87.5°C for wild-type enzyme | Caldococcus noboribetus |
Y309I/I310L/I321L/A325P/G326S | 50% inactivation after 10 min at 90°C as compared to 87.5°C for wild-type enzyme | Caldococcus noboribetus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Caldococcus noboribetus | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldococcus noboribetus | P96318 | - |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Caldococcus noboribetus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Caldococcus noboribetus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ICDH | - |
Caldococcus noboribetus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Caldococcus noboribetus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
74 | - |
10 min, 50% loss of activity, mutant enzyme A336F | Caldococcus noboribetus |
88 | - |
10 min, 50% loss of activity, wild-type enzyme and mutant enzyme Y309I/I310L | Caldococcus noboribetus |
89 | - |
10 min, 50% loss of activity, mutant enzyme I321L | Caldococcus noboribetus |
91 | - |
10 min, 50% loss of activity, mutant enzyme A325P/G326S | Caldococcus noboribetus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Caldococcus noboribetus |