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Literature summary for 1.1.1.42 extracted from

  • Iwabata, H.; Watanabe, K.; Ohkuri, T.; Yokobori, S.; Yamagishi, A.
    Thermostability of ancestral mutants of Caldococcus noboribetus isocitrate dehydrogenase (2005), FEMS Microbiol. Lett., 243, 393-398.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzymes are expressed in Escherichia coli Caldococcus noboribetus

Protein Variants

Protein Variants Comment Organism
A325P/G326S 50% inactivation after 10 min at 91.3°C as compared to 87.5°C for wild-type enzyme Caldococcus noboribetus
A336F 50% inactivation after 10 min at 74°C as compared to 87.5°C or wild-type enzyme Caldococcus noboribetus
I321L 50% inactivation after 10 min at 88.9°C as compared to 87.5°C for wild-type enzyme Caldococcus noboribetus
Y309I/I310L 50% inactivation after 10 min at 88.4°C as compared to 87.5°C for wild-type enzyme Caldococcus noboribetus
Y309I/I310L/I321L/A325P/G326S 50% inactivation after 10 min at 90°C as compared to 87.5°C for wild-type enzyme Caldococcus noboribetus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Caldococcus noboribetus
-
2-oxoglutarate + CO2 + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Caldococcus noboribetus P96318
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Caldococcus noboribetus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
Caldococcus noboribetus 2-oxoglutarate + CO2 + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
ICDH
-
Caldococcus noboribetus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Caldococcus noboribetus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
74
-
10 min, 50% loss of activity, mutant enzyme A336F Caldococcus noboribetus
88
-
10 min, 50% loss of activity, wild-type enzyme and mutant enzyme Y309I/I310L Caldococcus noboribetus
89
-
10 min, 50% loss of activity, mutant enzyme I321L Caldococcus noboribetus
91
-
10 min, 50% loss of activity, mutant enzyme A325P/G326S Caldococcus noboribetus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Caldococcus noboribetus