Cloned (Comment) | Organism |
---|---|
gene icd, sequence comparisons, expression of His6-tagged in Escherichia coli strain Rosetta (DE3) | Microcystis aeruginosa |
gene icd, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) | Microcystis aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Microcystis aeruginosa | |
Co2+ | - |
Microcystis aeruginosa | |
Cu2+ | complete inhibition | Microcystis aeruginosa | |
K+ | slight inhibition | Microcystis aeruginosa | |
Li+ | slight inhibition | Microcystis aeruginosa | |
Na+ | slight inhibition | Microcystis aeruginosa | |
Ni2+ | - |
Microcystis aeruginosa | |
Rb+ | slight inhibition | Microcystis aeruginosa | |
Zn2+ | strong inhibition | Microcystis aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis | Microcystis aeruginosa | |
0.0322 | - |
NADP+ | with Mg2+, pH 7.5, 37°C | Microcystis aeruginosa | |
0.0324 | - |
NADP+ | with Mg2+, pH 7.5, 50°C | Microcystis aeruginosa | |
0.0326 | - |
DL-isocitrate | with Mg2+, pH 7.5, 50°C | Microcystis aeruginosa | |
0.0326 | - |
DL-isocitrate | with Mg2+, pH 7.5, 37°C | Microcystis aeruginosa | |
0.0717 | - |
NADP+ | with Mn2+, pH 7.5, 50°C | Microcystis aeruginosa | |
0.0797 | - |
NADP+ | with Mn2+, pH 7.5, 37°C | Microcystis aeruginosa | |
0.1243 | - |
DL-isocitrate | with Mn2+, pH 7.5, 50°C | Microcystis aeruginosa | |
0.1243 | - |
DL-isocitrate | with Mn2+, pH 7.5, 37°C | Microcystis aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates poorly, 2.48% activity compared to Mn2+ | Microcystis aeruginosa | |
Co2+ | activates, 10.22% activity compared to Mn2+ | Microcystis aeruginosa | |
K+ | activates slightly, 10.18% activity compared to Mn2+ | Microcystis aeruginosa | |
Li+ | activates slightly, 12.63% activity compared to Mn2+ | Microcystis aeruginosa | |
Mg2+ | can partially substitute for Mn2+ | Microcystis aeruginosa | |
Mg2+ | activates, 72.45% activity compared to Mn2+ | Microcystis aeruginosa | |
Mn2+ | required | Microcystis aeruginosa | |
Mn2+ | activates, required, best divalent cation | Microcystis aeruginosa | |
additional information | the enzyme is completely divalent cation dependent. As compared with Mn2+, MaIDH shows about 2.5times and 4times higher affinities (1/Km) to NADP+ and DL-isocitrate with Mg2+, Cu2+ does not activate the enzyme at all | Microcystis aeruginosa | |
Na+ | activates slightly, 10.70% activity compared to Mn2+ | Microcystis aeruginosa | |
Ni2+ | activates poorly, 1.40% activity compared to Mn2+ | Microcystis aeruginosa | |
Rb+ | activates slightly, 20.67% activity compared to Mn2+ | Microcystis aeruginosa | |
Zn2+ | activates poorly, 4.23% activity compared to Mn2+ | Microcystis aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52600 | - |
- |
Microcystis aeruginosa |
52600 | - |
2 * 52600 | Microcystis aeruginosa |
105000 | - |
gel filtration | Microcystis aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-isocitrate + NADP+ | Microcystis aeruginosa | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
DL-isocitrate + NADP+ | Microcystis aeruginosa | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microcystis aeruginosa | - |
- |
- |
Microcystis aeruginosa | A8YGS2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by metal affinity chromatography | Microcystis aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-isocitrate + NADP+ | - |
Microcystis aeruginosa | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
DL-isocitrate + NADP+ | - |
Microcystis aeruginosa | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 52600 | Microcystis aeruginosa |
homodimer | 2 * 52600, SDS-PAGE | Microcystis aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
ICD | - |
Microcystis aeruginosa |
NADP+-dependent IDH | - |
Microcystis aeruginosa |
NADP+-dependent isocitrate dehydrogenase | - |
Microcystis aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
with Mg2+ | Microcystis aeruginosa |
45 | - |
with Mn2+ | Microcystis aeruginosa |
50 | - |
with Mg2+ | Microcystis aeruginosa |
50 | - |
with Mn2+ | Microcystis aeruginosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | activity range | Microcystis aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
purified recombinant enzyme, 20 min, stable | Microcystis aeruginosa |
40 | - |
purified recombinant enzyme, 20 min, loss of 50% of enzyme activity, with Mg2+ | Microcystis aeruginosa |
45 | - |
purified recombinant enzyme, 20 min, loss of 50% of enzyme activity, with Mn2+ | Microcystis aeruginosa |
45 | - |
purified enzyme, 20 min, 50% loss of enzyme activity | Microcystis aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.29 | - |
DL-isocitrate | with Mg2+, pH 7.5, 50°C | Microcystis aeruginosa | |
10.92 | - |
DL-isocitrate | with Mg2+, pH 7.5, 37°C | Microcystis aeruginosa | |
25.36 | - |
NADP+ | with Mg2+, pH 7.5, 50°C | Microcystis aeruginosa | |
25.36 | - |
NADP+ | with Mg2+, pH 7.5, 37°C | Microcystis aeruginosa | |
43.21 | - |
DL-isocitrate | with Mn2+, pH 7.5, 50°C | Microcystis aeruginosa | |
43.21 | - |
DL-isocitrate | with Mn2+, pH 7.5, 37°C | Microcystis aeruginosa | |
48.88 | - |
NADP+ | with Mn2+, pH 7.5, 50°C | Microcystis aeruginosa | |
48.88 | - |
NADP+ | with Mn2+, pH 7.5, 37°C | Microcystis aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
with Mn2+ or Mg2+ | Microcystis aeruginosa |
7.5 | - |
the recombinant MaIDH has a broader pH optimum from pH 6.5 to pH 8.5 | Microcystis aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | activity range | Microcystis aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | purified recombinant MaIDH is completely specific with NADP+, and no NAD+-dependent activity is detectable | Microcystis aeruginosa | |
NADP+ | the enzyme is completely NADP+-dependent and no NAD+-linked activity is detectable | Microcystis aeruginosa | |
NADPH | - |
Microcystis aeruginosa |
General Information | Comment | Organism |
---|---|---|
physiological function | isocitrate dehydrogenase of Microcystis aeruginosa plays important roles in energy and biosynthesis metabolisms and its catalytic product 2-oxoglutarate provides the carbon skeleton for ammonium assimilation and also constitutes a signaling molecule of nitrogen starvation in cyanobacteria | Microcystis aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00335 | - |
DL-isocitrate | with Mg2+, pH 7.5, 50°C | Microcystis aeruginosa | |
0.00348 | - |
DL-isocitrate | with Mn2+, pH 7.5, 50°C | Microcystis aeruginosa | |
0.00613 | - |
NADP+ | with Mn2+, pH 7.5, 50°C | Microcystis aeruginosa | |
0.00787 | - |
NADP+ | with Mg2+, pH 7.5, 50°C | Microcystis aeruginosa | |
335 | - |
DL-isocitrate | with Mg2+, pH 7.5, 37°C | Microcystis aeruginosa | |
348 | - |
DL-isocitrate | with Mn2+, pH 7.5, 37°C | Microcystis aeruginosa | |
613 | - |
NADP+ | with Mn2+, pH 7.5, 37°C | Microcystis aeruginosa | |
787 | - |
NADP+ | with Mg2+, pH 7.5, 37°C | Microcystis aeruginosa |