Cloned (Comment) | Organism |
---|---|
SdIDH, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) | Streptomyces diastaticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Streptomyces diastaticus | |
Co2+ | - |
Streptomyces diastaticus | |
Cu2+ | complete inhibition | Streptomyces diastaticus | |
EDTA | - |
Streptomyces diastaticus | |
K+ | - |
Streptomyces diastaticus | |
Na+ | - |
Streptomyces diastaticus | |
Zn2+ | - |
Streptomyces diastaticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates, about 50% activity compared to Mn2+, SdIDH displays a 19000-32000fold (kcat/Km) preference for NADP+ over NAD+ with Mn2+ and Mg2+, respectively | Streptomyces diastaticus | |
Mn2+ | activates, preferred cation, SdIDH displays a 19000-32000fold (kcat/Km) preference for NADP+ over NAD+ with Mn2+ and Mg2+, respectively | Streptomyces diastaticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
gel filtration | Streptomyces diastaticus |
80000 | - |
1 * 80000, SDS-PAGE | Streptomyces diastaticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-isocitrate + NADP+ | Streptomyces diastaticus | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
DL-isocitrate + NADP+ | Streptomyces diastaticus M1033 | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces diastaticus | - |
- |
- |
Streptomyces diastaticus M1033 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) | Streptomyces diastaticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-isocitrate + NAD+ | low activityy with NAD+ | Streptomyces diastaticus | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
DL-isocitrate + NAD+ | low activityy with NAD+ | Streptomyces diastaticus M1033 | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
DL-isocitrate + NADP+ | - |
Streptomyces diastaticus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
DL-isocitrate + NADP+ | NADP+ is the highly preferred cofactor | Streptomyces diastaticus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
DL-isocitrate + NADP+ | - |
Streptomyces diastaticus M1033 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
DL-isocitrate + NADP+ | NADP+ is the highly preferred cofactor | Streptomyces diastaticus M1033 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 80000, SDS-PAGE | Streptomyces diastaticus |
Synonyms | Comment | Organism |
---|---|---|
IDH | - |
Streptomyces diastaticus |
NADP+-dependent IDH | - |
Streptomyces diastaticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
with Mg2+ | Streptomyces diastaticus |
40 | - |
with Mn2+ | Streptomyces diastaticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | activity range, profile overview | Streptomyces diastaticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified enzyme, 20 min, stable up to 40°C | Streptomyces diastaticus |
47 | - |
purified enzyme, 20 min, loss of 50% activity | Streptomyces diastaticus |
50 | - |
purified enzyme, 20 min, inactivation | Streptomyces diastaticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
with Mn2+ | Streptomyces diastaticus |
9 | - |
with Mg2+ | Streptomyces diastaticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | activity range, profile overview | Streptomyces diastaticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | SdIDH displays a 19000-32000fold (kcat/Km) preference for NADP+ over NAD+ with Mn2+ and Mg2+, respectively | Streptomyces diastaticus | |
NAD+ | poor cofactor | Streptomyces diastaticus | |
NADP+ | dependent on | Streptomyces diastaticus |
General Information | Comment | Organism |
---|---|---|
metabolism | NAD(P)+-dependent isocitrate dehydrogenase is a key enzyme in tricarboxylic acid cycle | Streptomyces diastaticus |