Cloned (Comment) | Organism |
---|---|
gene Rv3339c, expression of N-terminally His6-tagged enzyme | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
purified ICDH-1 in complex with NADPH at Mn2+, hanging drop vapor diffusion method, 0.001 ml of protein in 5 mM NADPH, and 5 mM MnCl2 is mixed with 0.001 ml of reservoir solution containing 30% PEG 2000 and 0.1 M Tris-HCl, pH 8.0, room temperature, X-ray diffraction structure determination and analysis at 2.18 A resoltuion | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | noncompetitive versus NADP+ | Mycobacterium tuberculosis | |
malate | noncompetitive versus NADP+ and isocitrate | Mycobacterium tuberculosis | |
additional information | product inhibition patterns for ICDH-1, overview | Mycobacterium tuberculosis | |
NADPH | product inhibition competitive versus NADP+ and noncompetitive versus isocitrate | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, kinetic mechanism of ICDH-1, overview | Mycobacterium tuberculosis | |
0.015 | - |
NADP+ | pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme | Mycobacterium tuberculosis | |
0.05 | - |
isocitrate | pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Mycobacterium tuberculosis | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | Mycobacterium tuberculosis H37Rv | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WKL1 | gene Rv3339c | - |
Mycobacterium tuberculosis H37Rv | P9WKL1 | gene Rv3339c | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme by nickel affinity chromatography and gel filtration | Mycobacterium tuberculosis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH + H+ | protonation of the enolate to form product 2-oxoglutarate is the rate-limiting step | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Mycobacterium tuberculosis | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | - |
Mycobacterium tuberculosis H37Rv | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
additional information | Mycobacterium tuberculosis ICDH-1 also catalyzes the formation of 2-hydroxyglutarate | Mycobacterium tuberculosis | ? | - |
? | |
additional information | Mycobacterium tuberculosis ICDH-1 also catalyzes the formation of 2-hydroxyglutarate | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | each subunit has a Rossmann fold, and a common top domain of interlocking beta sheets | Mycobacterium tuberculosis |
More | three-dimensional structure of Mtb ICDH-1, overview | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
ICDH | - |
Mycobacterium tuberculosis |
ICDH-1 | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33 | - |
NADP+ | pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme | Mycobacterium tuberculosis | |
33 | - |
isocitrate | pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Mycobacterium tuberculosis |