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Literature summary for 1.1.1.42 extracted from

  • Lu, Q.; McAlister-Henn, L.
    Peroxisomal localization and function of NADP+-specific isocitrate dehydrogenases in yeast (2010), Arch. Biochem. Biophys., 493, 125-134.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged IDPA in Escherichia coli strain BL21(DE3), expression of IDPA inm Saccharomyces cerevisiae, it behaves similar to the yeast IDP2 harboring a type I peroxisomal targeting sequenceand occurs in cytosol and peroxisomes, complementation study in Saccharomyces cerevisiae disruption mutants expressing the full-length IDPA enzyme from Aspergillus nidulans, and IDPA lacking the mitochondrial targeting sequence and containing a different PTS1, which results in the same expression level and subcellular orientation, subcellular localization study, overview Aspergillus nidulans

Protein Variants

Protein Variants Comment Organism
additional information construction of IDP1, IDP2, and IDP3 disruption mutants, and of double and triple disruption mutants in haploid strain MMY011. Complementation study in disruption mutants expressing the full-length IDPA enzyme from Aspergillus nidulans, which behaves similar to the yeast IDP2 harboring a type I peroxisomal targeting sequence, PTS1, and occurs in cytosol and peroxisomes, subcellular localization study, overview. Expression of IDPA lacking the mitochondrial targeting sequence and containing a different PTS1 results in the same expression level and subcellular orientation Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Aspergillus nidulans 5829
-
cytosol IDP2 carrying a type I peroxisomal targeting sequence, IDP2+CKL, is only partially localized to peroxisomes, the enzyme is able to function either instead of peroxisomal IDP3 or as cytosolic IDP2 Saccharomyces cerevisiae 5829
-
mitochondrion IDP1 Saccharomyces cerevisiae 5739
-
additional information subcellular localization study of isozymes, overview Saccharomyces cerevisiae
-
-
peroxisome
-
Aspergillus nidulans 5777
-
peroxisome IDP3 contains a canonical type I peroxisomal targeting sequence, a carboxyl-terminal Cys-Lys-Leu tripeptide. IDP3 is normally strictly peroxisomal Saccharomyces cerevisiae 5777
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Saccharomyces cerevisiae
Mg2+ activates Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Saccharomyces cerevisiae
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+ Aspergillus nidulans
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+ Saccharomyces cerevisiae MMY011
-
2-oxoglutarate + NADPH + H+ + CO2
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
-
-
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae MMY011
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged IDPA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
Saccharomyces cerevisiae 2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+
-
Aspergillus nidulans 2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+
-
Saccharomyces cerevisiae MMY011 2-oxoglutarate + NADPH + H+ + CO2
-
?

Synonyms

Synonyms Comment Organism
IDP3
-
Saccharomyces cerevisiae
IDPA
-
Aspergillus nidulans
NADP+-specific isocitrate dehydrogenase
-
Saccharomyces cerevisiae
NADP+-specific isocitrate dehydrogenase
-
Aspergillus nidulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
IDP2 Saccharomyces cerevisiae
8
-
-
Aspergillus nidulans

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 80% of maximal activity in the pH range of pH 7.0-9.0 Aspergillus nidulans

Cofactor

Cofactor Comment Organism Structure
NADP+ specific for Saccharomyces cerevisiae
NADP+ specific for Aspergillus nidulans

pI Value

Organism Comment pI Value Maximum pI Value
Saccharomyces cerevisiae IDP2, isoelectric focusing
-
6.5
Aspergillus nidulans IDPA, isoelectric focusing
-
7.4
Saccharomyces cerevisiae IDP1, isoelectric focusing
-
8.2
Saccharomyces cerevisiae IDP3, isoelectric focusing
-
8.9

General Information

General Information Comment Organism
physiological function IDP3 provides the NADPH required for beta-oxidation of some fatty acids in the peroxisome Saccharomyces cerevisiae