Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C269S | activity of the C269S mutant is not affected by glutathione disulfide and no glutathionylated IDPc is observed with 5 mM glutathione disulfide confirming that Cys269 is a target of IDPc glutathionylation | Homo sapiens |
C379S | glutathionylation of the C379S mutant is similar to that of wild type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Diamide | - |
Homo sapiens | |
glutathione disulfide | incubation with 5 mM glutathione disulfide for 30 min completely eliminates activity | Homo sapiens | |
additional information | 5 mM glutathione does not noticeably inhibit IDPc activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glutathionylation | Cys269 of IDPc is a target for S-glutathionylation, this modification is reversed by dithiothreitol as well as enzymatically by cytosolic glutaredoxin in the presence of glutathione. Glutathionylated IDPc is significantly less susceptible than native protein to peptide fragmentation by reactive oxygen species and proteolytic digestion. Glutathionylation may play a protective role in the degradation of protein through the structural alterations of IDPc. | Homo sapiens |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Homo sapiens | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytosolic NADP+-dependent isocitrate dehydrogenase | - |
Homo sapiens |
ICDH | - |
Homo sapiens |
IDPc | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens |