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Literature summary for 1.1.1.42 extracted from

  • Rodriguez-Arnedo, A.; Camacho, M.; Llorca, F.; Bonete, M.J.
    Complete reversal of coenzyme specificity of isocitrate dehydrogenase from Haloferax volcanii (2005), Protein J., 24, 259-266.
    View publication on PubMed
Search for more literature for 1.1.1.42

Protein Variants

Protein Variants Comment Organism
R291S involved in coenzyme specificity Haloferax volcanii
R291S/K343D/Y344I/V350A/Y390P switch in coenzyme specificity from NADP+ to NAD+ Haloferax volcanii
R291S/K343D/Y344I/Y390P involved in coenzyme specificity Haloferax volcanii
R291S/K343D/Y390P involved in coenzyme specificity Haloferax volcanii
R291S/Y390P involved in coenzyme specificity Haloferax volcanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 DL-isocitrate mutant R291S/K343D/Y344I/V350A/Y390P, pH 8.0, 30°C Haloferax volcanii
0.018
-
 DL-isocitrate mutant R291S, pH 8.0, 30°C Haloferax volcanii
0.036
-
 DL-isocitrate mutant R291S/K343D/Y390P, pH 8.0, 30°C Haloferax volcanii
0.036
-
 DL-isocitrate mutant R291S/Y390P, pH 8.0, 30°C Haloferax volcanii
0.04
-
 DL-isocitrate mutant R291S/K343D/Y344I/Y390P, pH 8.0, 30°C Haloferax volcanii
0.101
-
 NADP+ wild-type, pH 8.0, 30°C Haloferax volcanii
0.108
-
 DL-isocitrate wild-type, pH 8.0, 30°C Haloferax volcanii
0.144
-
 NAD+ mutant R291S/K343D/Y344I/V350A/Y390P, pH 8.0, 30°C Haloferax volcanii
0.203
-
 NADP+ mutant R291S/K343D/Y390P, pH 8.0, 30°C Haloferax volcanii
0.282
-
 NADP+ mutant R291S/K343D/Y344I/Y390P, pH 8.0, 30°C Haloferax volcanii
0.327
-
 NADP+ mutant R291S/Y390P, pH 8.0, 30°C Haloferax volcanii
0.35
-
 NADP+ mutant R291S, pH 8.0, 30°C Haloferax volcanii

Organism

Organism UniProt Comment Textmining
Haloferax volcanii Q8X277 recombinant enzyme
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-isocitrate + NAD+ NAD+ is only substrate for mutant R291S/K343D/Y344I/V350A/Y390P Haloferax volcanii 2-oxoglutarate + CO2 + NADH
-
 ?
DL-isocitrate + NADP+ NAD+ is not substrate for mutant R291S/K343D/Y344I/V350A/Y390P Haloferax volcanii 2-oxoglutarate + CO2 + NADPH
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0003
-
 NADP+ mutant R291S/K343D/Y344I/Y390P, pH 8.0, 30°C Haloferax volcanii
0.0005
-
 DL-isocitrate mutant R291S/Y390P, pH 8.0, 30°C Haloferax volcanii
0.0007
-
 NAD+ mutant R291S/K343D/Y344I/V350A/Y390P, pH 8.0, 30°C Haloferax volcanii
0.001
-
 NADP+ mutant R291S/Y390P, pH 8.0, 30°C Haloferax volcanii
0.0013
-
 DL-isocitrate mutant R291S/K343D/Y390P, pH 8.0, 30°C Haloferax volcanii
0.002
-
 DL-isocitrate mutant R291S, pH 8.0, 30°C Haloferax volcanii
0.0023
-
 DL-isocitrate wild-type, pH 8.0, 30°C Haloferax volcanii
0.0024
-
 NADP+ mutant R291S/K343D/Y390P, pH 8.0, 30°C Haloferax volcanii
0.0025
-
 DL-isocitrate mutant R291S/K343D/Y344I/Y390P, pH 8.0, 30°C Haloferax volcanii
0.003
-
 NADP+ wild-type, pH 8.0, 30°C Haloferax volcanii
0.004
-
 NADP+ mutant R291S, pH 8.0, 30°C Haloferax volcanii
0.005
-
 DL-isocitrate mutant R291S/K343D/Y344I/V350A/Y390P, pH 8.0, 30°C Haloferax volcanii