Crystallization (Comment) | Organism |
---|---|
in complex with Mn2+ and isocitrate | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
N328D | 36% decrease in vmax-value compared to wild-type | Sus scrofa |
N328S | slight decrease in vmax-value compared to wild-type | Sus scrofa |
R83K | slight decrease in vmax-value compared to wild-type | Sus scrofa |
R83Q | slight decrease in vmax-value compared to wild-type | Sus scrofa |
T311A | slight decrease in vmax-value compared to wild-type | Sus scrofa |
T311N | vmax-value is less than 1% of the value of wild-type | Sus scrofa |
T311S | large increase in vmax-value compared to wild-type | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0043 | - |
NADP+ | mutant T311N, pH 7.4, 25°C | Sus scrofa | |
0.0056 | - |
NADP+ | wild-type, pH 7.4, 25°C | Sus scrofa | |
0.0064 | - |
DL-isocitrate | mutant T311S, pH 7.4, 25°C | Sus scrofa | |
0.0065 | - |
DL-isocitrate | mutant R83K, pH 7.4, 25°C | Sus scrofa | |
0.0073 | - |
DL-isocitrate | mutant T311N, pH 7.4, 25°C | Sus scrofa | |
0.0084 | - |
DL-isocitrate | wild-type, pH 7.4, 25°C | Sus scrofa | |
0.0097 | - |
NADP+ | mutant T311S, pH 7.4, 25°C | Sus scrofa | |
0.0114 | - |
DL-isocitrate | mutant N328D, pH 7.4, 25°C | Sus scrofa | |
0.014 | - |
DL-isocitrate | mutant R83Q, pH 7.4, 25°C | Sus scrofa | |
0.0154 | - |
DL-isocitrate | mutant N328S, pH 7.4, 25°C | Sus scrofa | |
0.0155 | - |
DL-isocitrate | mutant T311A, pH 7.4, 25°C | Sus scrofa | |
0.0203 | - |
NADP+ | mutant R83K, pH 7.4, 25°C | Sus scrofa | |
0.0246 | - |
NADP+ | mutant N328D, pH 7.4, 25°C | Sus scrofa | |
0.0749 | - |
NADP+ | mutant T311A, pH 7.4, 25°C | Sus scrofa | |
0.0811 | - |
NADP+ | mutant R83Q, pH 7.4, 25°C | Sus scrofa | |
0.119 | - |
NADP+ | mutant N328S, pH 7.4, 25°C | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Sus scrofa | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | wild-type, Km-value 0.00033 mM | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | P33198 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-isocitrate + NADP+ | - |
Sus scrofa | 2-oxoglutarate + CO2 + NADPH | - |
? |