Protein Variants | Comment | Organism |
---|---|---|
D375N | 15fold increase in KM-value for NADP+, marked decrease of Vmax-value | Sus scrofa |
K260Q | 28fold increase in KM-value for NADP+, marked decrease of Vmax-value | Sus scrofa |
K374Q | little change in kinetic parameters | Sus scrofa |
T373A | reduction of Vmax-value to 1% of wild-type | Sus scrofa |
T373S | little change in kinetic parameters | Sus scrofa |
T373V | reduction of Vmax-value to 20% of wild-type | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0055 | - |
NADP+ | mutant T373S, 25°C, pH 7.4 | Sus scrofa | |
0.0062 | - |
NADP+ | mutant T373A, 25°C, pH 7.4 | Sus scrofa | |
0.0088 | - |
NADP+ | wild-type, 25°C, pH 7.4 | Sus scrofa | |
0.012 | - |
NADP+ | mutant T373V, 25°C, pH 7.4 | Sus scrofa | |
0.0154 | - |
NADP+ | mutant K374Q 25°C, pH 7.4 | Sus scrofa | |
0.0179 | - |
NAD+ | wild-type, 25°C, pH 7.4 | Sus scrofa | |
0.0207 | - |
NAD+ | mutant K260R, 25°C, pH 7.4 | Sus scrofa | |
0.0232 | - |
NAD+ | mutant D375N, 25°C, pH 7.4 | Sus scrofa | |
0.0312 | - |
NAD+ | mutant K260Q, 25°C, pH 7.4 | Sus scrofa | |
0.0378 | - |
NADP+ | mutant K260R, 25°C, pH 7.4 | Sus scrofa | |
0.133 | - |
NADP+ | mutant D375N, 25°C, pH 7.4 | Sus scrofa | |
0.25 | - |
NADP+ | mutant K260Q, 25°C, pH 7.4 | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | P33198 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-isocitrate + NAD+ | - |
Sus scrofa | 2-oxoglutarate + CO2 + NADH | - |
? | |
DL-isocitrate + NADP+ | - |
Sus scrofa | 2-oxoglutarate + CO2 + NADPH | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
NAD+ | mutant D375N, 25°C, pH 7.4 | Sus scrofa | |
0.47 | - |
NAD+ | mutant K260Q, 25°C, pH 7.4 | Sus scrofa | |
0.5 | - |
NAD+ | mutant K260R, 25°C, pH 7.4 | Sus scrofa | |
0.76 | - |
NAD+ | wild-type, 25°C, pH 7.4 | Sus scrofa | |
1.7 | - |
NADP+ | mutant K260Q, 25°C, pH 7.4 | Sus scrofa | |
5.7 | - |
NADP+ | mutant K260R, 25°C, pH 7.4 | Sus scrofa | |
8.4 | - |
NADP+ | mutant D375N, 25°C, pH 7.4 | Sus scrofa | |
32.2 | - |
NADP+ | wild-type, 25°C, pH 7.4 | Sus scrofa |