Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cis-aconitate | isozyme ICDH2, not isozyme ICDH1 | Spirodela polyrhiza | |
citrate | isozymes ICDH2 and ICDH1 | Spirodela polyrhiza | |
glutamate | 160% stimulation of isozyme ICDH1 at 0.3 mM | Spirodela polyrhiza | |
isocitrate | isozymes ICDH2 and ICDH1 | Spirodela polyrhiza | |
additional information | no effect by trans-aconitate on isozyme ICDH2 | Spirodela polyrhiza |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | isozymes ICDH2 and ICDH1, isozyme ICDH2 is more sensitive to negative feedback inhibition by 2-oxoglutarate | Spirodela polyrhiza | |
cis-aconitate | isozyme ICDH1, not isozyme ICDH2 | Spirodela polyrhiza | |
glutamate | 80% inhibition of isozyme ICDH2 at 2 mM | Spirodela polyrhiza | |
succinate | isozymes ICDH2 and ICDH1 | Spirodela polyrhiza | |
trans-aconitate | isozyme ICDH1, not isozyme ICDH2 | Spirodela polyrhiza |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
isocitrate | chloroplastic isozyme ICDH2, pH 7.8, 25°C | Spirodela polyrhiza | |
0.08 | - |
NADP+ | chloroplastic isozyme ICDH2, pH 7.8, 25°C | Spirodela polyrhiza | |
0.08 | - |
Mn2+ | chloroplastic isozyme ICDH2, pH 7.8, 25°C | Spirodela polyrhiza | |
0.12 | - |
Mg2+ | chloroplastic isozyme ICDH2, pH 7.8, 25°C | Spirodela polyrhiza | |
0.17 | - |
NADP+ | cytosolic isozyme ICDH1, pH 7.8, 25°C | Spirodela polyrhiza | |
0.18 | - |
isocitrate | cytosolic isozyme ICDH1, pH 7.8, 25°C | Spirodela polyrhiza | |
0.21 | - |
Mn2+ | cytosolic isozyme ICDH1, pH 7.8, 25°C | Spirodela polyrhiza | |
0.31 | - |
Mg2+ | cytosolic isozyme ICDH1, pH 7.8, 25°C | Spirodela polyrhiza |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isozyme ICDH2, minor form | Spirodela polyrhiza | 9507 | - |
cytosol | isozyme ICDH1, major form | Spirodela polyrhiza | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Spirodela polyrhiza | |
Mn2+ | - |
Spirodela polyrhiza |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Spirodela polyrhiza | metabolic regulation of the isozymes | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spirodela polyrhiza | - |
duck weed, isozymes ICDH1 and ICDH2 | - |
Purification (Comment) | Organism |
---|---|
isozymes ICDH1, 172fold, and ICDH2, 31fold, by ammonium sulfate fractionation, desalting, DEAE ion exchange and gel filtration | Spirodela polyrhiza |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0046 | - |
purified chloroplastic isozyme ICDH1 | Spirodela polyrhiza |
0.038 | - |
purified cytosolic isozyme ICDH1 | Spirodela polyrhiza |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Spirodela polyrhiza | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | metabolic regulation of the isozymes | Spirodela polyrhiza | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ICDH | - |
Spirodela polyrhiza |
NADP-dependent isocitrate dehydrogenase | - |
Spirodela polyrhiza |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Spirodela polyrhiza |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 7.9 | isozymes ICDH2 and ICDH1 | Spirodela polyrhiza |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Spirodela polyrhiza |