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Literature summary for 1.1.1.42 extracted from

  • Popova, T.N.; Rakhmanova, T.I.; Appenroth, K.J.
    Cytosolic and chloroplastic NADP-dependent isocitrate dehydrogenases in Spirodela polyrhiza. I. Regulation of activity by metabolites in vitro (2002), J. Plant Physiol., 159, 231-237.
No PubMed abstract available
Search for more literature for 1.1.1.42

Activating Compound

Activating Compound Comment Organism Structure
cis-aconitate isozyme ICDH2, not isozyme ICDH1 Spirodela polyrhiza
citrate isozymes ICDH2 and ICDH1 Spirodela polyrhiza
glutamate 160% stimulation of isozyme ICDH1 at 0.3 mM Spirodela polyrhiza
isocitrate isozymes ICDH2 and ICDH1 Spirodela polyrhiza
additional information no effect by trans-aconitate on isozyme ICDH2 Spirodela polyrhiza

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate isozymes ICDH2 and ICDH1, isozyme ICDH2 is more sensitive to negative feedback inhibition by 2-oxoglutarate Spirodela polyrhiza
cis-aconitate isozyme ICDH1, not isozyme ICDH2 Spirodela polyrhiza
glutamate 80% inhibition of isozyme ICDH2 at 2 mM Spirodela polyrhiza
succinate isozymes ICDH2 and ICDH1 Spirodela polyrhiza
trans-aconitate isozyme ICDH1, not isozyme ICDH2 Spirodela polyrhiza

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 isocitrate chloroplastic isozyme ICDH2, pH 7.8, 25°C Spirodela polyrhiza
0.08
-
 NADP+ chloroplastic isozyme ICDH2, pH 7.8, 25°C Spirodela polyrhiza
0.08
-
 Mn2+ chloroplastic isozyme ICDH2, pH 7.8, 25°C Spirodela polyrhiza
0.12
-
 Mg2+ chloroplastic isozyme ICDH2, pH 7.8, 25°C Spirodela polyrhiza
0.17
-
 NADP+ cytosolic isozyme ICDH1, pH 7.8, 25°C Spirodela polyrhiza
0.18
-
 isocitrate cytosolic isozyme ICDH1, pH 7.8, 25°C Spirodela polyrhiza
0.21
-
 Mn2+ cytosolic isozyme ICDH1, pH 7.8, 25°C Spirodela polyrhiza
0.31
-
 Mg2+ cytosolic isozyme ICDH1, pH 7.8, 25°C Spirodela polyrhiza

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast isozyme ICDH2, minor form Spirodela polyrhiza 9507
-
cytosol isozyme ICDH1, major form Spirodela polyrhiza 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Spirodela polyrhiza
Mn2+
-
 Spirodela polyrhiza

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Spirodela polyrhiza metabolic regulation of the isozymes 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Spirodela polyrhiza
-
 duck weed, isozymes ICDH1 and ICDH2
-

Purification (Commentary)

Purification (Comment) Organism
isozymes ICDH1, 172fold, and ICDH2, 31fold, by ammonium sulfate fractionation, desalting, DEAE ion exchange and gel filtration Spirodela polyrhiza

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0046
-
 purified chloroplastic isozyme ICDH1 Spirodela polyrhiza
0.038
-
 purified cytosolic isozyme ICDH1 Spirodela polyrhiza

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
 Spirodela polyrhiza 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
isocitrate + NADP+ metabolic regulation of the isozymes Spirodela polyrhiza 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
ICDH
-
 Spirodela polyrhiza
NADP-dependent isocitrate dehydrogenase
-
 Spirodela polyrhiza

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Spirodela polyrhiza

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 7.9 isozymes ICDH2 and ICDH1 Spirodela polyrhiza

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Spirodela polyrhiza