Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression of the cDNA in a construct of nucleotides 1-1714 with rat PEPCK promotor and the SV-40 polyadenylation signal, permanant expression in 3T3-L1 cells and in transgenic mice, the latter are constructed by infection of fertilized eggs | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transgenic mice by infection of fertilized eggs, enzyme overexpressing transgenic mice show increased triglyceride and cholesterol levels and develop fatty liver, hyperlipidemia, and obesity | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Mus musculus | cytosolic enzyme is critical in fat and cholesterol biosynthesis, enzyme content correlates with adipogenesis in wild-type adipocytes and in transgenic cells | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
26-weeks-old male mice | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipocyte | - |
Mus musculus | - |
adipose tissue | - |
Mus musculus | - |
epididymis | epididymal fat pad | Mus musculus | - |
fat pad | epididymal fat pad, hepatic fat pad | Mus musculus | - |
liver | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.6 | - |
wild-type epidydymal fat pad | Mus musculus |
50 | - |
transgenic mice epidydymal fat pad | Mus musculus |
77.4 | - |
wild-type liver | Mus musculus |
108.2 | - |
transgenic mice liver | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | cytosolic enzyme is critical in fat and cholesterol biosynthesis, enzyme content correlates with adipogenesis in wild-type adipocytes and in transgenic cells | Mus musculus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
threo-Ds-isocitrate + NADP+ | - |
Mus musculus | 2-oxoglutarate + CO2 + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDPc | - |
Mus musculus |
NADP+-dependent isocitrate dehydrogenase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | essential | Mus musculus |