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Literature summary for 1.1.1.42 extracted from

  • Kanao, T.; Kawamura, M.; Fukui, T.; Atomi, H.; Imanaka, T.
    Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola. A carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle (2002), Eur. J. Biochem., 269, 1926-1931.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
construction of a genomic cDNA library, gene Cl-idh, DNA sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) Chlorobium limicola

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition of carboxylation by citrate, pyruvate, succinate, fumarate, malate, glyoxylate, ATP, and ADP Chlorobium limicola
oxaloacetate competitive, inhibits the reverse reaction, over 50% inhibition at 1 mM, synergistically with glyoxylate Chlorobium limicola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
NADP+ recombinant enyme, pH 9.0 Chlorobium limicola
0.045
-
isocitrate recombinant enyme, pH 9.0 Chlorobium limicola
1.1
-
2-oxoglutarate recombinant enyme, pH 7.0 Chlorobium limicola
1.3
-
CO2 recombinant enyme, pH 7.0 Chlorobium limicola

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
native PAGE Chlorobium limicola
81000
-
gel filtration Chlorobium limicola

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Chlorobium limicola carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle, preference for carboxylation reaction direction 2-oxoglutarate + CO2 + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Chlorobium limicola
-
green sulfur bacterium, strain M1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by ion exchange and gel filtration to homogeneity, 1.84fold Chlorobium limicola

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36
-
purified recombinant enzyme Chlorobium limicola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
Chlorobium limicola 2-oxoglutarate + CO2 + NADPH + H+
-
r
isocitrate + NADP+ carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle, preference for carboxylation reaction direction Chlorobium limicola 2-oxoglutarate + CO2 + NADPH + H+
-
r

Subunits

Subunits Comment Organism
monomer 1 * 80000 Chlorobium limicola

Synonyms

Synonyms Comment Organism
isocitrate dehydrogenase
-
Chlorobium limicola
NADP-dependent IDH
-
Chlorobium limicola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
reverse reaction Chlorobium limicola
9
-
forward reaction Chlorobium limicola

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADH Chlorobium limicola
NADP+
-
Chlorobium limicola
NADPH
-
Chlorobium limicola

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.35
-
oxaloacetate recombinant enzyme, pH 7.0 Chlorobium limicola