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Literature summary for 1.1.1.42 extracted from
- Two structurally different NADP-specific isocitrate dehydrogenases in an obligately psychrophilic bacterium, Vibrio sp. strain ABE-1 (1984), J. Gen. Appl. Microbiol., 30, 479-487.
No PubMed abstract available
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolute requirement for divalent cations | Vibrio sp. |  |
| Mn2+ | absolute requirement for divalent cations | Vibrio sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
gel filtration, isoenzyme I: dimer | Vibrio sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NADP+ | Halobacterium salinarum | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
| isocitrate + NADP+ | Vibrio sp. | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
| isocitrate + NADP+ | Vibrio sp. ABE-1 | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halobacterium salinarum | - |
- |
- |
| Vibrio sp. | - |
- |
- |
| Vibrio sp. ABE-1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| strain ABE-1 | Vibrio sp. |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| with 2-mercaptoethanol after inactivation at 40°C, isoenzyme II, 40% renaturation | Vibrio sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NADP+ | - |
Halobacterium salinarum | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
| isocitrate + NADP+ | - |
Vibrio sp. | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
| isocitrate + NADP+ | - |
Vibrio sp. ABE-1 | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | isoenzyme I | Vibrio sp. |
| monomer | isoenzyme II, 1 * 85000, SDS-PAGE | Vibrio sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
isoenzyme II | Vibrio sp. |
| 40 | - |
isoenzyme I | Vibrio sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | - |
isoenzyme II | Vibrio sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Halobacterium salinarum | |
| NADP+ | - |
Vibrio sp. | |
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Release 2023.1 (January 2023)
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