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Literature summary for 1.1.1.41 extracted from

  • McAlister-Henn, L.
    Ligand binding and structural changes associated with allostery in yeast NAD+-specific isocitrate dehydrogenase (2012), Arch. Biochem. Biophys., 519, 112-117.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
AMP allosteric activation, binds to subunit IDH1 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C150S site-directed mutagenesis of subunit IDH2, disulfide bridge formation by C150 is abolished Saccharomyces cerevisiae
C56S/C242S site-directed mutagenesis of subunit IDH2, the mutation does not affect disulfide formation in the enzyme Saccharomyces cerevisiae
D279A site-directed mutagenesis of subunit IDH1, the mutation results in a loss of activation by AMP Saccharomyces cerevisiae
D286A site-directed mutagenesis of subunit IDH2, the mutation results in a dramatic reduction in Vmax primarily due to a 70fold increase in the S0.5 value for NAD+ Saccharomyces cerevisiae
G15D site-directed mutagenesis of subunit IDH1, the tetrameric IDH1G15D/IDH2 enzyme exhibits half-site binding (two sites) for isocitrate in the absence of DTT and full-site binding (four sites) in the presence of DTT Saccharomyces cerevisiae
I280A site-directed mutagenesis of subunit IDH1, the mutation results in a loss of activation by AMP Saccharomyces cerevisiae
I287A site-directed mutagenesis of subunit IDH2, the mutation results in a dramatic reduction in Vmax primarily due to a 70fold increase in the S0.5 value for NAD+ Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NAD+ Saccharomyces cerevisiae
-
2-oxoglutarate + CO2 + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NAD+
-
Saccharomyces cerevisiae 2-oxoglutarate + CO2 + NADH + H+
-
?

Subunits

Subunits Comment Organism
More subunit IDH2 contains catalytic isocitrate/Mg2+ and NAD+ binding sites whereas subunit IDH1 contains homologous binding sites, respectively, for cooperative binding of isocitrate and for allosteric binding of AMP. The subsunits share 42% sequence identity Saccharomyces cerevisiae
octamer 4 * regulatory IDH1 + 4 * catalytic IDH2 subunits Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
NAD+-specific isocitrate dehydrogenase
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Saccharomyces cerevisiae

General Information

General Information Comment Organism
metabolism yeast IDH is regulated both by allostery and by covalent formation of a disulfide bond, and these regulatory mechanisms contribute to modulation of respiratory metabolism in vivo Saccharomyces cerevisiae