Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.41 extracted from

  • Miyazaki, K.
    Bifunctional isocitrate-homoisocitrate dehydrogenase: a missing link in the evolution of beta-decarboxylating dehydrogenase (2005), Biochem. Biophys. Res. Commun., 331, 341-346.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21-CodonPlus (DE3)-RIL Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0164
-
isocitrate 6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1mM NAD+ Pyrococcus horikoshii
0.0183
-
homoisocitrate 6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1mM NAD+ Pyrococcus horikoshii
0.0771
-
NAD+ 6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2 Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
Pyrococcus horikoshii
Mn2+
-
Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Hi-Trap chelating HP column chromatography Pyrococcus horikoshii

Renatured (Commentary)

Renatured (Comment) Organism
using N-lauroylsarcosine to 0.3% v/v Pyrococcus horikoshii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
no activity on 3-isopropylmalate Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-homoisocitrate + NAD+
-
Pyrococcus horikoshii ? + NADH
-
?
D-isocitrate + NAD+
-
Pyrococcus horikoshii 2-oxoglutarate + CO2 + NADH
-
?
isocitrate + NAD+
-
Pyrococcus horikoshii 2-oxoglutarate + CO2 + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
beta-decarboxylating dehydrogenase
-
Pyrococcus horikoshii
isocitrate-homoisocitrate dehydrogenase
-
Pyrococcus horikoshii
PH1722
-
Pyrococcus horikoshii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.7
-
homoisocitrate 6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1 mM NAD+ Pyrococcus horikoshii
14.8
-
isocitrate 6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1 mM NAD+ Pyrococcus horikoshii
21.8
-
NAD+ 6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2 Pyrococcus horikoshii

Cofactor

Cofactor Comment Organism Structure
NAD+ 1 mM Pyrococcus horikoshii