Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21-CodonPlus (DE3)-RIL | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0164 | - |
isocitrate | 6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1mM NAD+ | Pyrococcus horikoshii | |
0.0183 | - |
homoisocitrate | 6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1mM NAD+ | Pyrococcus horikoshii | |
0.0771 | - |
NAD+ | 6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2 | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Pyrococcus horikoshii | |
Mn2+ | - |
Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Hi-Trap chelating HP column chromatography | Pyrococcus horikoshii |
Renatured (Comment) | Organism |
---|---|
using N-lauroylsarcosine to 0.3% v/v | Pyrococcus horikoshii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
no activity on 3-isopropylmalate | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-homoisocitrate + NAD+ | - |
Pyrococcus horikoshii | ? + NADH | - |
? | |
D-isocitrate + NAD+ | - |
Pyrococcus horikoshii | 2-oxoglutarate + CO2 + NADH | - |
? | |
isocitrate + NAD+ | - |
Pyrococcus horikoshii | 2-oxoglutarate + CO2 + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
beta-decarboxylating dehydrogenase | - |
Pyrococcus horikoshii |
isocitrate-homoisocitrate dehydrogenase | - |
Pyrococcus horikoshii |
PH1722 | - |
Pyrococcus horikoshii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.7 | - |
homoisocitrate | 6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1 mM NAD+ | Pyrococcus horikoshii | |
14.8 | - |
isocitrate | 6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1 mM NAD+ | Pyrococcus horikoshii | |
21.8 | - |
NAD+ | 6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2 | Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | 1 mM | Pyrococcus horikoshii |