Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | allosteric activator, binding site at subunit IDH1 | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in yeast strain IDH12DELTAL deficient in both subunits of the enzyme | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D279A/D280A | site-directed mutagenesis, mutation of a IDH1 residue, reduced AMP binding, ligand-binding analysis | Saccharomyces cerevisiae |
D286A/I287A | site-directed mutagenesis, mutation of a IDH2 residue, highly reduced NAD+ binding, ligand-binding analysis | Saccharomyces cerevisiae |
H281A | site-directed mutagenesis, mutation of a IDH2 residue, mutant cannot bind NAD+, ligand-binding analysis | Saccharomyces cerevisiae |
R274A | site-directed mutagenesis, mutation of a IDH1 residue, reduced AMP binding, ligand-binding analysis | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and ligand binding | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | binding site at subunit IDH2, active site | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
303000 | - |
sequence calculation | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | Saccharomyces cerevisiae | enzyme performs the rate-limiting step in the mitochondrial tricarboxylic cycle and is subject to complex allosteric regulation | 2-oxoglutarate + CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from strain IDH12DELTAL by nickel affinity chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-isocitrate + NAD+ | catalytic residues binding isocitrate are located on subunit IDH2, while regulatory residues binding isocitrate are located on subunit IDH1 | Saccharomyces cerevisiae | 2-oxoglutarate + CO2 + NADH | - |
? | |
isocitrate + NAD+ | enzyme performs the rate-limiting step in the mitochondrial tricarboxylic cycle and is subject to complex allosteric regulation | Saccharomyces cerevisiae | 2-oxoglutarate + CO2 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | alpha4beta4, subunits are termed IDH1 and IDH2 | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
IDH | - |
Saccharomyces cerevisiae |
isocitrate dehydrogenase | - |
Saccharomyces cerevisiae |
NAD+-specific isocitrate dehydrogenase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | specific for, binding site at subunit IDH2 | Saccharomyces cerevisiae |