Crystallization (Comment) | Organism |
---|---|
crystallization in artificial mother liquor supplemented with 100 mM NAD+ | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C201M/C332Y/K344D/Y345I/V351A/Y391K/R395S | converts the cofactor specificity from 7000-fold preference of NADP+ to a 200-fold preference of NAD+ | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
NADP+ | wild type | Escherichia coli | |
0.099 | - |
NAD+ | 7-fold mutant | Escherichia coli | |
4.7 | - |
NAD+ | wild type | Escherichia coli | |
5.8 | - |
NADP+ | 7-fold mutant | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | Escherichia coli | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | - |
Escherichia coli | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NADP+ | - |
Escherichia coli | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
NADP+ | - |
Escherichia coli |