Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | activates ICDH-1 | Phycomyces blakesleeanus |
General Stability | Organism |
---|---|
ICDH-2 is more stable to urea than ICDH-1 | Phycomyces blakesleeanus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | inhibits ICDH-2 | Phycomyces blakesleeanus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, active substrate is Mg-DL-isocitrate chelate | Phycomyces blakesleeanus | |
Mn2+ | requires divalent cations | Phycomyces blakesleeanus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40500 | - |
alpha8, 8 * 40500, SDS PAGE, ICDH-1 and ICDH-2 | Phycomyces blakesleeanus |
338000 | - |
gel filtration | Phycomyces blakesleeanus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | Phycomyces blakesleeanus | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phycomyces blakesleeanus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Phycomyces blakesleeanus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37.5 | - |
ICDH-1 | Phycomyces blakesleeanus |
48.9 | - |
ICDH-2 | Phycomyces blakesleeanus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | - |
Phycomyces blakesleeanus | 2-oxoglutarate + CO2 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | alpha8, 8 * 40500, SDS PAGE, ICDH-1 and ICDH-2 | Phycomyces blakesleeanus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | - |
Phycomyces blakesleeanus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
Phycomyces blakesleeanus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Phycomyces blakesleeanus |