Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Cereibacter sphaeroides |
Protein Variants | Comment | Organism |
---|---|---|
additional information | directed functional immobilization of the enzyme on gold surfaces, representing a proof-of-concept for the development of reactors for electrochemical synthon preparation using dehydrogenases, method development and evaluation, overview | Cereibacter sphaeroides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26385 | - |
4 * 26385, amino acid sequence calculation | Cereibacter sphaeroides |
110000 | - |
- |
Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
galactitol + NAD+ | Cereibacter sphaeroides | - |
L-tagatose + NADH + H+ | - |
r | |
galactitol + NAD+ | Cereibacter sphaeroides D | - |
L-tagatose + NADH + H+ | - |
r | |
additional information | Cereibacter sphaeroides | GatDH from Rhodobacter sphaeroides is a multifunctional enzyme that catalyzes in the presence of oxidized beta-NAD+ the interconversion of various multivalent aliphatic alcohols to the corresponding ketone. GatDH oxidizes a variety of polyvalent aliphatic alcohols and polyols to the corresponding ketones and ketoses, respectively, and in the reverse reaction, it reduces ketones with high stereoselectivity yielding the corresponding S-configurated alcohols | ? | - |
? | |
additional information | Cereibacter sphaeroides D | GatDH from Rhodobacter sphaeroides is a multifunctional enzyme that catalyzes in the presence of oxidized beta-NAD+ the interconversion of various multivalent aliphatic alcohols to the corresponding ketone. GatDH oxidizes a variety of polyvalent aliphatic alcohols and polyols to the corresponding ketones and ketoses, respectively, and in the reverse reaction, it reduces ketones with high stereoselectivity yielding the corresponding S-configurated alcohols | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | C0KTJ6 | - |
- |
Cereibacter sphaeroides D | C0KTJ6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-hexanediol + NAD+ | with the redox mediator 4-carboxy-2,5,7-trinitrofluorenylidenmalonnitrile, CTFM, in solute form | Cereibacter sphaeroides | 2-oxohexanol + NADH + H+ | - |
? | |
1,2-hexanediol + NAD+ | with the redox mediator 4-carboxy-2,5,7-trinitrofluorenylidenmalonnitrile, CTFM, in solute form | Cereibacter sphaeroides D | 2-oxohexanol + NADH + H+ | - |
? | |
galactitol + NAD+ | - |
Cereibacter sphaeroides | L-tagatose + NADH + H+ | - |
r | |
galactitol + NAD+ | - |
Cereibacter sphaeroides D | L-tagatose + NADH + H+ | - |
r | |
additional information | GatDH from Rhodobacter sphaeroides is a multifunctional enzyme that catalyzes in the presence of oxidized beta-NAD+ the interconversion of various multivalent aliphatic alcohols to the corresponding ketone. GatDH oxidizes a variety of polyvalent aliphatic alcohols and polyols to the corresponding ketones and ketoses, respectively, and in the reverse reaction, it reduces ketones with high stereoselectivity yielding the corresponding S-configurated alcohols | Cereibacter sphaeroides | ? | - |
? | |
additional information | GatDH from Rhodobacter sphaeroides is a multifunctional enzyme that catalyzes in the presence of oxidized beta-NAD+ the interconversion of various multivalent aliphatic alcohols to the corresponding ketone. GatDH oxidizes a variety of polyvalent aliphatic alcohols and polyols to the corresponding ketones and ketoses, respectively, and in the reverse reaction, it reduces ketones with high stereoselectivity yielding the corresponding S-configurated alcohols | Cereibacter sphaeroides D | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 26385, amino acid sequence calculation | Cereibacter sphaeroides |
Synonyms | Comment | Organism |
---|---|---|
galactitol dehydrogenase | - |
Cereibacter sphaeroides |
GatDH | - |
Cereibacter sphaeroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cereibacter sphaeroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cereibacter sphaeroides | |
NADH | - |
Cereibacter sphaeroides |