Cloned (Comment) | Organism |
---|---|
overexpression of TarI and TarJ individually from their respective genes using the T7 expression system | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide | - |
Haemophilus influenzae | |
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide | - |
Staphylococcus aureus | |
D-ribitol 5-phosphate | - |
Haemophilus influenzae | |
D-ribitol 5-phosphate | - |
Staphylococcus aureus | |
NADP+ | competitive versus NADPH | Haemophilus influenzae | |
NADP+ | competitive versus NADPH | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00674 | - |
NADPH | - |
Staphylococcus aureus | |
0.00706 | - |
NADPH | - |
Haemophilus influenzae | |
0.0285 | - |
ribulose 5-phosphate | - |
Staphylococcus aureus | |
0.106 | - |
ribulose 5-phosphate | - |
Haemophilus influenzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
2 * 26000 + 2 * 41000, tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex, SDS-PAGE | Staphylococcus aureus |
41000 | - |
2 * 26000 + 2 * 41000, tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex, SDS-PAGE | Staphylococcus aureus |
53190 | - |
2 * 53190, calculation from amino acid composition | Haemophilus influenzae |
106400 | - |
amino acid composition | Haemophilus influenzae |
129000 | - |
calculation from gel filtration and sedimentation velocity data | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | - |
- |
- |
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate + NADPH + H+ | tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex. Release and free diffusion of the intermediate ribitol 5-phosphate without channelling between active sites, ordered bi-bi mechanism | Staphylococcus aureus | D-ribitol 5-phosphate + NADP+ | - |
? | |
D-ribulose 5-phosphate + NADPH + H+ | the data suggest that NADPH binds first and ribulose 5-phosphate binds second. Bifunction enzyme catalyzing the reactions of EC 1.1.1.137 and EC 2.7.7.40, ordered bi-bi mechanism | Haemophilus influenzae | D-ribitol 5-phosphate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 2 * 26000 + 2 * 41000, tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex, SDS-PAGE | Staphylococcus aureus |
dimer | 2 * 53190, calculation from amino acid composition | Haemophilus influenzae |
Synonyms | Comment | Organism |
---|---|---|
Bcs1 | - |
Haemophilus influenzae |
TarIJ | - |
Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | 0.55 | NADPH | - |
Haemophilus influenzae | |
0.79 | - |
NADPH | - |
Staphylococcus aureus | |
6.08 | - |
NADPH | - |
Staphylococcus aureus | |
7.41 | - |
NADPH | - |
Haemophilus influenzae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide | Ki(intercept) and Ki(slope)-values | Staphylococcus aureus | |
additional information | - |
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide | Ki(intercept) and Ki(slope)-values | Haemophilus influenzae |