Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Sulfobacillus thermosulfidooxidans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 5 mM, 73% residual activity; 73.7% residual activity at 5 mM EDTA | Sulfobacillus thermosulfidooxidans | |
EGTA | 5 mM, 72% residual activity; 72.9% residual activity at 5 mM EGTA | Sulfobacillus thermosulfidooxidans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
NAD+ | pH 9.5, 50°C | Sulfobacillus thermosulfidooxidans | |
0.42 | - |
NAD+ | at pH 9.5 and 50°C | Sulfobacillus thermosulfidooxidans | |
2 | - |
2-dehydro-3-deoxy-L-rhamnonate | pH 9.5, 50°C | Sulfobacillus thermosulfidooxidans | |
2 | - |
2-dehydro-3-deoxy-L-rhamnonate | at pH 9.5 and 50°C | Sulfobacillus thermosulfidooxidans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, 118% of initial activity | Sulfobacillus thermosulfidooxidans | |
Co2+ | 118.6% activity at 5 mM | Sulfobacillus thermosulfidooxidans | |
additional information | activity is not significantly affected by the addition of 5 mM metal ions | Sulfobacillus thermosulfidooxidans | |
additional information | the enzyme activity is not significantly affected by the addition of 5 mM Ca2+, Zn2+, Mg2+, Mn2+, Fe2+, Fe3+, and Ni2+ | Sulfobacillus thermosulfidooxidans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-3-deoxy-L-rhamnonate + NAD+ | Sulfobacillus thermosulfidooxidans | - |
2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+ | - |
? | |
2-dehydro-3-deoxy-L-rhamnonate + NAD+ | Sulfobacillus thermosulfidooxidans AT-1 | - |
2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfobacillus thermosulfidooxidans | - |
- |
- |
Sulfobacillus thermosulfidooxidans | P0DOW0 | - |
- |
Sulfobacillus thermosulfidooxidans AT-1 | P0DOW0 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Q-Sepharose column chromatography | Sulfobacillus thermosulfidooxidans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.78 | - |
unpurified enzyme, at pH 9.0 and 50°C | Sulfobacillus thermosulfidooxidans |
4.06 | - |
pH 9.5, 50°C | Sulfobacillus thermosulfidooxidans |
4.06 | - |
after 5.1fold purification, at pH 9.0 and 50°C | Sulfobacillus thermosulfidooxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-butanediol + NAD+ | the enzyme also shows weak 2,3-butanediol dehydrogenase activity (11.3% compared to 2-dehydro-3-deoxy-L-rhamnonate) | Sulfobacillus thermosulfidooxidans | ? | - |
? | |
2,3-butanediol + NAD+ | the enzyme also shows weak 2,3-butanediol dehydrogenase activity (11.3% compared to 2-dehydro-3-deoxy-L-rhamnonate) | Sulfobacillus thermosulfidooxidans AT-1 | ? | - |
? | |
2-dehydro-3-deoxy-L-rhamnonate + NAD+ | - |
Sulfobacillus thermosulfidooxidans | 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+ | - |
? | |
2-dehydro-3-deoxy-L-rhamnonate + NAD+ | 100% activity | Sulfobacillus thermosulfidooxidans | 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+ | - |
? | |
2-dehydro-3-deoxy-L-rhamnonate + NAD+ | - |
Sulfobacillus thermosulfidooxidans AT-1 | 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+ | - |
? | |
2-dehydro-3-deoxy-L-rhamnonate + NAD+ | 100% activity | Sulfobacillus thermosulfidooxidans AT-1 | 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+ | - |
? | |
additional information | enzyme shows low activity towards 2,3-butanediol (11% of the activity with 2-dehydro-3-deoxy-L-rhamnonate), and very little activity towards L-rhamnonate, 2-dehydro-3-deoxy-L-mannonate, L-lyxonate and L-mannonate. No substrates are: aldoses such as D-glucose, D-mannose, D-galactose, D-fructose, D-xylose, D-fucose, D-arabinose, D-lyxose, D-ribose, D-fucose, L-fucose, L-xylose, L-arabinose, L-sorbose, polyols such as sorbitol, mannitol, xylitol, amino acids such as L-threonine, L-serine, alcohols such as methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 1,2-propandiol, 1,3-propandiol, aldonates such as D-gluconate, D-galactonate, D-mannonate, D-arabinonate, D-lyxonate, D-xylonate, organic acids such as pyruvate, D-lactate, L-lactate, aldehydes such as D-lactaldehyde, L-lactaldehyde, acetaldehyde, DL-glyceraldehyde, glycolaldehyde | Sulfobacillus thermosulfidooxidans | ? | - |
? | |
additional information | the enzyme shows less than 5% activity with L-rhamnonate, 2-dehydro-3-deoxy-L-mannonate, L-lyxonate, and L-mannonate. The enzyme does not show any detectable activity in the presence of NADP+ | Sulfobacillus thermosulfidooxidans | ? | - |
? | |
additional information | the enzyme shows less than 5% activity with L-rhamnonate, 2-dehydro-3-deoxy-L-mannonate, L-lyxonate, and L-mannonate. The enzyme does not show any detectable activity in the presence of NADP+ | Sulfobacillus thermosulfidooxidans AT-1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-dehydro-3-deoxy-L-rhamnonate dehydrogenase | - |
Sulfobacillus thermosulfidooxidans |
2-keto-3-deoxy-L-rhamnonate dehydrogenase | - |
Sulfobacillus thermosulfidooxidans |
KDRDH | - |
Sulfobacillus thermosulfidooxidans |
L-KDR dehydrogenase | - |
Sulfobacillus thermosulfidooxidans |
Sulth 3557 protein | - |
Sulfobacillus thermosulfidooxidans |
Sulth_3557 | - |
Sulfobacillus thermosulfidooxidans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Sulfobacillus thermosulfidooxidans |
60 | - |
- |
Sulfobacillus thermosulfidooxidans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | after 20 min incubation, the enzyme is stable at temperatures below 60°C, maintains 53.8% activity at 70°C, and is almost completely inactive at 80°C | Sulfobacillus thermosulfidooxidans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
20 min, stable | Sulfobacillus thermosulfidooxidans |
70 | - |
20 min, 53% residual activity | Sulfobacillus thermosulfidooxidans |
80 | - |
20 min, inactive | Sulfobacillus thermosulfidooxidans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Sulfobacillus thermosulfidooxidans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11.5 | little or no activity below pH 7.0, at pH 11.5 the enzme shows 75% of the activity at pH 9.5 | Sulfobacillus thermosulfidooxidans |
8 | 11 | the enzyme has no or little activity at pH below 7.0, about 50% activity at pH 8.0, while the enzyme activity at pH 11.0 is about 75% of that obtained at pH 9.5 | Sulfobacillus thermosulfidooxidans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no detectable activity with NADP+ | Sulfobacillus thermosulfidooxidans | |
NAD+ | - |
Sulfobacillus thermosulfidooxidans |