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Literature summary for 1.1.1.401 extracted from

  • Watanabe, S.; Makino, K.
    Novel modified version of nonphosphorylated sugar metabolism-an alternative L-rhamnose pathway of Sphingomonas sp (2009), FEBS J., 276, 1554-1567.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Sphingomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.646
-
2-dehydro-3-deoxy-L-rhamnonate at pH 8.0 and 25°C Sphingomonas sp.
1.27
-
2-dehydro-3-deoxy-L-lyxonate at pH 8.0 and 25°C Sphingomonas sp.
1.9
-
2-dehydro-3-deoxy-L-mannonate at pH 8.0 and 25°C Sphingomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25700
-
-
Sphingomonas sp.
30000
-
x * 30000, SDS-PAGE Sphingomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydro-3-deoxy-L-rhamnonate + NAD+ Sphingomonas sp.
-
2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
-
?
2-dehydro-3-deoxy-L-rhamnonate + NAD+ Sphingomonas sp. SKA58
-
2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Sphingomonas sp. Q1NEI6
-
-
Sphingomonas sp. SKA58 Q1NEI6
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Sphingomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-L-lyxonate + NAD+
-
Sphingomonas sp. 2,4-didehydro-3-deoxy-L-lyxonate + NADH + H+
-
?
2-dehydro-3-deoxy-L-lyxonate + NAD+
-
Sphingomonas sp. SKA58 2,4-didehydro-3-deoxy-L-lyxonate + NADH + H+
-
?
2-dehydro-3-deoxy-L-mannonate + NAD+
-
Sphingomonas sp. 2,4-didehydro-3-deoxy-L-mannonate + NADH + H+
-
?
2-dehydro-3-deoxy-L-mannonate + NAD+
-
Sphingomonas sp. SKA58 2,4-didehydro-3-deoxy-L-mannonate + NADH + H+
-
?
2-dehydro-3-deoxy-L-rhamnonate + NAD+
-
Sphingomonas sp. 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
-
?
2-dehydro-3-deoxy-L-rhamnonate + NAD+
-
Sphingomonas sp. SKA58 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Sphingomonas sp.
? x * 25700, calculated from amino acid sequence Sphingomonas sp.

Synonyms

Synonyms Comment Organism
L-2-keto-3-deoxyrhamnonate-(4)-dehydrogenase
-
Sphingomonas sp.
LKDR 4-dehydrogenase
-
Sphingomonas sp.
LRA5
-
Sphingomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
2-dehydro-3-deoxy-L-mannonate at pH 8.0 and 25°C Sphingomonas sp.
0.18
-
2-dehydro-3-deoxy-L-lyxonate at pH 8.0 and 25°C Sphingomonas sp.
0.86
-
2-dehydro-3-deoxy-L-rhamnonate at pH 8.0 and 25°C Sphingomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Sphingomonas sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0063
-
2-dehydro-3-deoxy-L-mannonate at pH 8.0 and 25°C Sphingomonas sp.
0.145
-
2-dehydro-3-deoxy-L-lyxonate at pH 8.0 and 25°C Sphingomonas sp.
1.35
-
2-dehydro-3-deoxy-L-rhamnonate at pH 8.0 and 25°C Sphingomonas sp.