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Literature summary for 1.1.1.40 extracted from
- Biochemical properties and physiological roles of NADP-dependent malic enzyme in Escherichia coli (2011), J. Microbiol., 49, 797-802.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene maeB, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | deletion of gene maeB by chromosomal homologous recombination | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Escherichia coli |  |
| Co2+ | - |
Escherichia coli | |
| Cu2+ | strong inhibition | Escherichia coli | |
| Zn2+ | strong inhibition | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates, can partially substitute for Mn2+ to about 92% of the maximal activity | Escherichia coli | |
| Mn2+ | dependent on, best at 20 mM | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | - |
x * 83000, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | Escherichia coli | - |
pyruvate + CO2 + NADPH | - |
r | |
| (S)-malate + NADP+ | Escherichia coli MG1655 | - |
pyruvate + CO2 + NADPH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P76558 | gene maeB | - |
| Escherichia coli MG1655 | P76558 | gene maeB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.15 | - |
pH 7.8, 46°C, purified recombinant enzyme | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | - |
Escherichia coli | pyruvate + CO2 + NADPH | - |
r | |
| (S)-malate + NADP+ | - |
Escherichia coli MG1655 | pyruvate + CO2 + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 83000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MaeB | - |
Escherichia coli |
| NADP-dependent malic enzyme | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 46 | - |
- |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 55 | activity range, profile overview | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 10.5 | activity range, profile overview | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | dependent on | Escherichia coli | |
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