Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates | Cyprinus carpio | |
Mn2+ | activates | Salmo salar | |
Mn2+ | activates | Clarias gariepinus | |
Mn2+ | activates | Clupea harengus | |
Mn2+ | activates | Salmo trutta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
4 * 64000, SDS-PAGE | Clupea harengus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Cyprinus carpio | - |
pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | Salmo salar | - |
pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | Clarias gariepinus | - |
pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | Clupea harengus | - |
pyruvate + CO2 + NADPH | - |
? | |
(S)-malate + NADP+ | Clupea harengus | - |
pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | Salmo trutta | - |
pyruvate + CO2 + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clarias gariepinus | - |
- |
- |
Clupea harengus | - |
- |
- |
Clupea harengus | - |
from the Baltic Sea | - |
Cyprinus carpio | - |
- |
- |
Salmo salar | - |
- |
- |
Salmo trutta | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by anion exchange and affinity chromatography | Clupea harengus |
native NADP-specific malic enzyme by anion exchange and 2'5'-ADP affinity chromatography | Clupea harengus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spermatozoon | two molecular forms of malic enzyme are present in carp spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) | Cyprinus carpio | - |
spermatozoon | two molecular forms of malic enzyme are present in catfish spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) | Clarias gariepinus | - |
spermatozoon | two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) | Clupea harengus | - |
spermatozoon | two molecular forms of malic enzyme are present in salmon spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) | Salmo salar | - |
spermatozoon | two molecular forms of malic enzyme are present in salmon spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) | Salmo trutta | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.008 | - |
purified native enzyme, pH 7.5, 25°C | Clarias gariepinus |
0.023 | - |
purified native enzyme, pH 7.5, 25°C | Salmo trutta |
0.026 | - |
purified native enzyme, pH 7.5, 25°C | Salmo salar |
0.045 | - |
purified native enzyme, pH 7.5, 25°C | Cyprinus carpio |
0.213 | - |
purified native enzyme, pH 7.5, 25°C | Clupea harengus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Cyprinus carpio | pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | - |
Salmo salar | pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | - |
Clarias gariepinus | pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | - |
Clupea harengus | pyruvate + CO2 + NADPH | - |
? | |
(S)-malate + NADP+ | - |
Clupea harengus | pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | - |
Salmo trutta | pyruvate + CO2 + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 64000, SDS-PAGE | Clupea harengus |
tetramer | 4 * 64000, SDS-PAGE | Clupea harengus |
Synonyms | Comment | Organism |
---|---|---|
NADP-specific ME | - |
Clupea harengus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Cyprinus carpio |
25 | - |
assay at | Salmo salar |
25 | - |
assay at | Clarias gariepinus |
25 | - |
assay at | Clupea harengus |
25 | - |
assay at | Salmo trutta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
malate decarboxylation | Clupea harengus |
7.5 | - |
assay at | Cyprinus carpio |
7.5 | - |
assay at | Salmo salar |
7.5 | - |
assay at | Clarias gariepinus |
7.5 | - |
assay at | Clupea harengus |
7.5 | - |
assay at | Salmo trutta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | activity range, profile overview | Clupea harengus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Cyprinus carpio | |
NADP+ | - |
Salmo salar | |
NADP+ | - |
Clarias gariepinus | |
NADP+ | - |
Clupea harengus | |
NADP+ | - |
Salmo trutta | |
NADP+ | dependent on | Clupea harengus | |
NADPH | - |
Cyprinus carpio | |
NADPH | - |
Salmo salar | |
NADPH | - |
Clarias gariepinus | |
NADPH | - |
Clupea harengus | |
NADPH | - |
Salmo trutta |
General Information | Comment | Organism |
---|---|---|
metabolism | fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme | Cyprinus carpio |
metabolism | fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme | Salmo salar |
metabolism | fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme | Clarias gariepinus |
metabolism | fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme | Clupea harengus |
metabolism | fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme | Salmo trutta |