Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D139A | dimeric mutant enzyme with reduced activity compared to the wild type enzyme | Homo sapiens |
D568A | dimeric or tetrameric mutant enzyme with increased activity compared to the wild type enzyme | Homo sapiens |
D90A | dimeric or tetrameric mutant enzyme with reduced activity compared to the wild type enzyme | Homo sapiens |
H142A | dimeric mutant enzyme with increased activity compared to the wild type enzyme | Homo sapiens |
H142A/D568A | dimeric mutant enzyme with reduced activity compared to the wild type enzyme | Homo sapiens |
H51A | dimeric or tetrameric mutant enzyme with wild type activity | Homo sapiens |
H51A/D139A | dimeric or tetrameric mutant enzyme with reduced activity compared to the wild type enzyme | Homo sapiens |
H51A/D90A | dimeric or tetrameric mutant enzyme with reduced activity compared to the wild type enzyme | Homo sapiens |
W572A | dimeric mutant enzyme with slightly reduced activity compared to the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
NADP+ | mutant enzyme H51A/D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
0.0039 | - |
NADP+ | mutant enzyme D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
0.004 | - |
NADP+ | mutant enzyme H142A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
0.004 | - |
NADP+ | mutant enzyme H51A/D139A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
0.0042 | - |
NADP+ | mutant enzyme H142A/D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
0.0048 | - |
NADP+ | mutant enzyme D139A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
0.005 | - |
NADP+ | mutant enzyme D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
0.005 | - |
NADP+ | mutant enzyme W572A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
0.0072 | - |
NADP+ | mutant enzyme H51A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
0.009 | - |
NADP+ | wild type enzyme, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
0.49 | - |
(S)-malate | wild type enzyme, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
0.68 | - |
(S)-malate | mutant enzyme D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
0.84 | - |
(S)-malate | mutant enzyme H51A/D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
0.89 | - |
(S)-malate | mutant enzyme H142A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
0.92 | - |
(S)-malate | mutant enzyme H142A/D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
0.95 | - |
(S)-malate | mutant enzyme D139A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
0.97 | - |
(S)-malate | mutant enzyme D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
0.98 | - |
(S)-malate | mutant enzyme W572A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
1.03 | - |
(S)-malate | mutant enzyme H51A/D139A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
1.1 | - |
(S)-malate | mutant enzyme H51A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA-Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Homo sapiens | pyruvate + CO2 + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | the dimeric form of c-NADP-ME is as active as tetramers, analytical ultracentrifugation | Homo sapiens |
tetramer | c-NADP-ME exists mainly as a tetramer, analytical ultracentrifugation | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
c-NADP-ME | - |
Homo sapiens |
cytosolic NADP+-dependent malic enzyme | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
84.83 | - |
(S)-malate | mutant enzyme H51A/D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
94.57 | - |
(S)-malate | mutant enzyme D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
95.53 | - |
(S)-malate | mutant enzyme D139A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
98.03 | - |
(S)-malate | mutant enzyme H142A/D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
105.3 | - |
(S)-malate | mutant enzyme H51A/D139A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
106 | - |
(S)-malate | mutant enzyme W572A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
109.5 | - |
(S)-malate | mutant enzyme H51A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
110.9 | - |
(S)-malate | wild type enzyme, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
122.7 | - |
(S)-malate | mutant enzyme H142A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens | |
136.9 | - |
(S)-malate | mutant enzyme D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens |