Literature summary for 1.1.1.40 extracted from

Gerrard Wheeler, M.C.; Arias, C.L.; Maurino, V.G.; Andreo, C.S.; Drincovich, M.F.
Identification of domains involved in the allosteric regulation of cytosolic Arabidopsis thaliana NADP-malic enzymes (2009), FEBS J., 276, 5665-5677.

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Activating Compound

Activating Compound	Comment	Organism	Structure
fumarate	about 4fold increase of activity in the presence of 7.5 mM fumarate	Arabidopsis thaliana
succinate	about 3fold increase of activity in the presence of 7.5 mM fumarate	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
acetyl-CoA	-	Arabidopsis thaliana
aspartate	-	Arabidopsis thaliana
ATP	-	Arabidopsis thaliana
D-Glucose-6-phosphate	-	Arabidopsis thaliana
fumarate	-	Arabidopsis thaliana
oxaloacetate	-	Arabidopsis thaliana
succinate	-	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0065	-	NADP+	pH 7.5	Arabidopsis thaliana
0.072	-	NADP+	pH 7.5	Arabidopsis thaliana
0.5	-	pyruvate	pH 7.0	Arabidopsis thaliana
0.8	-	(S)-malate	pH 7.5	Arabidopsis thaliana
3.3	-	(S)-malate	pH 7.5	Arabidopsis thaliana
5	-	pyruvate	pH 7.0	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Arabidopsis thaliana	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
65000	-	SDS-PAGE	Arabidopsis thaliana
243000	-	gel filtration	Arabidopsis thaliana
249000	-	gel filtration	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9LYG3	-	-
Arabidopsis thaliana	Q9XGZ0	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-malate + NADP+	-	Arabidopsis thaliana	pyruvate + CO2 + NADPH	-	r

Synonyms

Synonyms	Comment	Organism
NADP-malic enzyme	-	Arabidopsis thaliana
NADP-ME2	isoform	Arabidopsis thaliana
NADP-ME3	isoform	Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
75	-	pyruvate	pH 7.0	Arabidopsis thaliana
237	-	pyruvate	pH 7.0	Arabidopsis thaliana
268	-	(S)-malate	pH 7.5	Arabidopsis thaliana
324	-	(S)-malate	pH 7.5	Arabidopsis thaliana

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
7.5	-	at pH 7.5 isoenzyme NADP-ME2 retains 95% of the maximal activity	Arabidopsis thaliana
7.5	-	at pH 7.5 isoenzyme NADP-ME3 retains 95% of the maximal activity	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)