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Literature summary for 1.1.1.40 extracted from

  • Fahnenstich, H.; Saigo, M.; Niessen, M.; Zanor, M.I.; Andreo, C.S.; Fernie, A.R.; Drincovich, M.F.; Fluegge, U.I.; Maurino, V.G.
    Alteration of organic acid metabolism in Arabidopsis overexpressing the maize C4 NADP-malic enzyme causes accelerated senescence during extended darkness (2007), Plant Physiol., 145, 640-652.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of the maize enzyme in Arabidopsis thaliana under control of the cauliflower mosaic virus 35S promoter Zea mays

Protein Variants

Protein Variants Comment Organism
additional information transgenic Arabidopsis thaliana plants overexpressing the maize C4 NADP-malic enzyme show 6-33fold increase enzyme activity and a more rapid progression of dark-induced senescence compared to the wild type plants, overview Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Zea mays 9507
-
additional information the recombinant enzyme is imported into chloroplasts of Spinacia oleracea Zea mays
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
4 * 62000, recombinant mature isozyme NADP-ME2, SDS-PAGE Zea mays
240000
-
recombinant isozyme NADP-ME2, gel filtration Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Zea mays the C4 isozyme takes part in the C4 and CAM photosynthetic metabolisms, overview pyruvate + CO2 + NADPH
-
r
additional information Zea mays diurnal effects of an enhanced chloroplastic NADP-ME activity on metabolite levels, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Zea mays pyruvate + CO2 + NADPH
-
r
(S)-malate + NADP+ the C4 isozyme takes part in the C4 and CAM photosynthetic metabolisms, overview Zea mays pyruvate + CO2 + NADPH
-
r
additional information diurnal effects of an enhanced chloroplastic NADP-ME activity on metabolite levels, overview Zea mays ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 62000, recombinant mature isozyme NADP-ME2, SDS-PAGE Zea mays

Synonyms

Synonyms Comment Organism
C4 NADP-malic enzyme
-
Zea mays

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Zea mays
NADPH
-
Zea mays