Cloned (Comment) | Organism |
---|---|
gene malE, DNA and amino acid sequence determination and analysis, genomic library construction, screening, and functional enzyme expression in Escherichia coli | Mucor circinelloides |
gene malE, DNA and amino acid sequence determination and analysis, genomic library construction, screening, and functional enzyme expression in Escherichia coli | Mortierella alpina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Mucor circinelloides | the enzyme is rate-limiting step for fatty acid biosynthesis in oleaginous fungi in which the extent of lipid accumulation is below the maximum possible, overview | pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | Mortierella alpina | the enzyme is rate-limiting step for fatty acid biosynthesis in oleaginous fungi in which the extent of lipid accumulation is below the maximum possible, overview | pyruvate + CO2 + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mortierella alpina | A6XP71 | a commercially useful oil-producing fungus, strain CBS 696.70, gene malE | - |
Mucor circinelloides | A6XP72 | a commercially useful oil-producing fungus, strain CBS 108.16, gene malE | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Mucor circinelloides | pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | - |
Mortierella alpina | pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | the enzyme is rate-limiting step for fatty acid biosynthesis in oleaginous fungi in which the extent of lipid accumulation is below the maximum possible, overview | Mucor circinelloides | pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | the enzyme is rate-limiting step for fatty acid biosynthesis in oleaginous fungi in which the extent of lipid accumulation is below the maximum possible, overview | Mortierella alpina | pyruvate + CO2 + NADPH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
malic enzyme | - |
Mucor circinelloides |
malic enzyme | - |
Mortierella alpina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mucor circinelloides |
7.5 | - |
assay at | Mortierella alpina |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Mucor circinelloides | |
NADP+ | - |
Mortierella alpina | |
NADPH | - |
Mucor circinelloides | |
NADPH | - |
Mortierella alpina |