Cloned (Comment) | Organism |
---|---|
gene maeB, overexpression in wild-type strain W3110, and in strains deficient for pyruvate formate lyase, lactate dehydrogenase, and glucose phosphotransferase, overview | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | effects of enzyme overexpression on the concentration of CO2 and the C4 metabolsim in wild-type strain and strains overexpressing other anaeplerotic enzymes, phenotypes, overview | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates | Escherichia coli | |
NH4Cl | activates | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Escherichia coli | the reaction plays a role in the C4 metabolism, pathway regulation, overview | pyruvate + CO2 + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene maeB | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
below, wild-type strain W3110 | Escherichia coli |
1.69 | - |
MaeB overexpressed in an anaplerotic enzyme overexpressing strain | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Escherichia coli | pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | the reaction plays a role in the C4 metabolism, pathway regulation, overview | Escherichia coli | pyruvate + CO2 + NADPH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
MaeB | - |
Escherichia coli |
NADP-dependent malic enzyme | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | specific for | Escherichia coli | |
NADPH | specific for | Escherichia coli |