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Literature summary for 1.1.1.40 extracted from

  • Chang, S.C.; Lin, K.Y.; Chen, Y.J.; Lai, C.H.; Chang, G.G.; Chou, W.Y.
    Critical roles of conserved carboxylic acid residues in pigeon cytosolic NADP+-dependent malic enzyme (2006), FEBS J., 273, 4072-4081.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes expressed in Escherichia coli Columba livia

Protein Variants

Protein Variants Comment Organism
D235A turnover number is 783.5fold lower than wild-type value Columba livia
D257A turnover number is 28.5fold lower than wild-type value Columba livia
D258A turnover number is 522fold lower than wild-type value Columba livia
E234A turnover number is 1.3fold higher than wild-type value Columba livia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00179
-
NADP+ pH 7.4, 25°C, mutant enzyme D235A Columba livia
0.0018
-
NADP+ pH 7.4, 25°C, mutant enzyme E234A Columba livia
0.00207
-
NADP+ pH 7.4, 25°C, Mn2+-activated, wild-type enzyme Columba livia
0.00283
-
NADP+ pH 7.4, 25°C, mutant enzyme D257A Columba livia
0.00296
-
NADP+ pH 7.4, 25°C, mutant enzyme D258A Columba livia
0.05
-
(S)-malate pH 7.4, 25°C, mutant enzyme D258A Columba livia
0.08
-
(S)-malate pH 7.4, 25°C, Mn2+-activated, wild-type enzyme Columba livia
0.1
-
(S)-malate pH 7.4, 25°C, mutant enzyme D235A Columba livia
0.1
-
(S)-malate pH 7.4, 25°C, mutant enzyme D257A Columba livia
0.27
-
(S)-malate pH 7.4, 25°C, Mg2+-activated, wild-type enzyme Columba livia
13.33
-
(S)-malate pH 7.4, 25°C, mutant enzyme E234A Columba livia

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates, KM: 0.16 mM for wild-type enzyme Columba livia
Mn2+ activates, KM: 0.00378 mM for wild-type enzyme Columba livia

Organism

Organism UniProt Comment Textmining
Columba livia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant mutant enzymes Columba livia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Columba livia pyruvate + CO2 + NADPH
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
(S)-malate pH 7.4, 25°C, mutant enzyme D235A Columba livia
0.005
-
(S)-malate pH 7.4, 25°C, mutant enzyme E234A Columba livia
0.006
-
(S)-malate pH 7.4, 25°C, mutant enzyme D258A Columba livia
0.3
-
(S)-malate pH 7.4, 25°C, Mg2+-activated, wild-type enzyme Columba livia
1.1
-
(S)-malate pH 7.4, 25°C, mutant enzyme D257A Columba livia
15
-
(S)-malate pH 7.4, 25°C, Mn2+-activated, wild-type enzyme Columba livia
31.34
-
(S)-malate pH 7.4, 25°C, Mn2+-activated, wild-type enzyme Columba livia
34.83
-
(S)-malate pH 7.4, 25°C, Mg2+-activated, wild-type enzyme Columba livia
46.44
-
(S)-malate pH 7.4, 25°C, mutant enzyme E234A Columba livia

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Columba livia