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Literature summary for 1.1.1.40 extracted from

  • Jernejc, K.; Legisa, M.
    The influence of metal ions on malic enzyme activity and lipid synthesis in Aspergillus niger (2002), FEMS Microbiol. Lett., 217, 185-190.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ complete inhibition at 0.1 mM, competitive to Mg2+ and Mn2+, enzyme inhibition leads to reduced lipid biosynthesis and accumulation of citric acid, quantitative overview Aspergillus niger
Fe2+
-
Aspergillus niger
Zn2+
-
Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mn2+ or Mg2+ required Aspergillus niger
Mn2+ Mn2+ or Mg2+ required Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aspergillus niger the enzyme is a supplier of reducing power in form of reduced NADPH for lipid biosynthesis ?
-
?
additional information Aspergillus niger NRRL 2270 the enzyme is a supplier of reducing power in form of reduced NADPH for lipid biosynthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger NRRL 2270
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is a supplier of reducing power in form of reduced NADPH for lipid biosynthesis Aspergillus niger ?
-
?
additional information the enzyme is a supplier of reducing power in form of reduced NADPH for lipid biosynthesis Aspergillus niger NRRL 2270 ?
-
?

Synonyms

Synonyms Comment Organism
malic enzyme
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Aspergillus niger

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01
-
Cu2+ pH 7.4, 30°C, with Mg2+ Aspergillus niger
0.045
-
Cu2+ pH 7.4, 30°C, with Mn2+ Aspergillus niger