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Literature summary for 1.1.1.40 extracted from

  • Rutter, W.J.; Lardy, H.A.
    Purification and properties of pigeon liver malic enzyme (1958), J. Biol. Chem., 233, 374-382.
    View publication on PubMed

General Stability

General Stability Organism
malic acid decreases heat stability at pH values above 5.0 Pigeon
Mg2+ or Mn2+ increase heat stability Pigeon

Inhibitors

Inhibitors Comment Organism Structure
adenosine 2'-phosphate
-
Pigeon
ADP
-
Pigeon
arsenite weak Pigeon
Cu2+ 3 mM, weak Pigeon
diphenyliodonium chloride weak Pigeon
Hg2+ 0.0005 mM, almost complete inhibition Pigeon
iodoacetate weak Pigeon
NAD+ weak Pigeon
o-Iodosobenzoate strong Pigeon
p-mercuribenzoate strong Pigeon

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
NADP+ pH 7.5 Pigeon
0.041
-
L-malate pH 6.5 Pigeon
0.39
-
L-malate pH 7.5 Pigeon
3.3
-
L-malate pH 8.5 Pigeon

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ less effective activation than Mn2+ Pigeon
Cu2+ weak activation Pigeon
Mg2+ promotes activity Pigeon
Mn2+ 0.004 mM, 50% of the maximal activity of the malic activity. 0.2 mM, 50% of the maximal activity of oxaloacetic decarboxylase Pigeon
Ni2+ weak activation Pigeon
Zn2+ weak activation Pigeon

Organism

Organism UniProt Comment Textmining
Pigeon
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pigeon

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Pigeon
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Pigeon

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+ the singly-ionized species is the substrate, doubly-ionized malate is unreactive Pigeon pyruvate + CO2 + NADPH
-
r
Oxaloacetate
-
Pigeon Pyruvate + CO2
-
?
pyruvate + CO2 + NADPH
-
Pigeon L-malate + NADP+
-
r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
Mg2+ or Mn2+ increase heat stability Pigeon
additional information
-
malic acid decreases heat stability at pH values above 5.0 Pigeon

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
in presence of 0.1 mM malate Pigeon
8.5 9 in presence of 0.1 M malate Pigeon

Cofactor

Cofactor Comment Organism Structure
NADP+ cofactor Pigeon
NADP+ stimulation of decarboxylation of oxaloacetate Pigeon
NADPH cofactor Pigeon