General Stability | Organism |
---|---|
malic acid decreases heat stability at pH values above 5.0 | Pigeon |
Mg2+ or Mn2+ increase heat stability | Pigeon |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 2'-phosphate | - |
Pigeon | |
ADP | - |
Pigeon | |
arsenite | weak | Pigeon | |
Cu2+ | 3 mM, weak | Pigeon | |
diphenyliodonium chloride | weak | Pigeon | |
Hg2+ | 0.0005 mM, almost complete inhibition | Pigeon | |
iodoacetate | weak | Pigeon | |
NAD+ | weak | Pigeon | |
o-Iodosobenzoate | strong | Pigeon | |
p-mercuribenzoate | strong | Pigeon |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
NADP+ | pH 7.5 | Pigeon | |
0.041 | - |
L-malate | pH 6.5 | Pigeon | |
0.39 | - |
L-malate | pH 7.5 | Pigeon | |
3.3 | - |
L-malate | pH 8.5 | Pigeon |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | less effective activation than Mn2+ | Pigeon | |
Cu2+ | weak activation | Pigeon | |
Mg2+ | promotes activity | Pigeon | |
Mn2+ | 0.004 mM, 50% of the maximal activity of the malic activity. 0.2 mM, 50% of the maximal activity of oxaloacetic decarboxylase | Pigeon | |
Ni2+ | weak activation | Pigeon | |
Zn2+ | weak activation | Pigeon |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pigeon | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pigeon |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Pigeon | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pigeon |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | the singly-ionized species is the substrate, doubly-ionized malate is unreactive | Pigeon | pyruvate + CO2 + NADPH | - |
r | |
Oxaloacetate | - |
Pigeon | Pyruvate + CO2 | - |
? | |
pyruvate + CO2 + NADPH | - |
Pigeon | L-malate + NADP+ | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Mg2+ or Mn2+ increase heat stability | Pigeon |
additional information | - |
malic acid decreases heat stability at pH values above 5.0 | Pigeon |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
in presence of 0.1 mM malate | Pigeon |
8.5 | 9 | in presence of 0.1 M malate | Pigeon |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | cofactor | Pigeon | |
NADP+ | stimulation of decarboxylation of oxaloacetate | Pigeon | |
NADPH | cofactor | Pigeon |