Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DMSO | the addition of 10% (v/v) dimethyl sulfoxide increases the activity by 161.2% | Rhodococcus erythropolis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhodococcus erythropolis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | 38.3% residual activity at 10% (v/v) | Rhodococcus erythropolis | |
acetonitrile | 2.4% residual activity at 10% (v/v) | Rhodococcus erythropolis | |
Ag+ | complete inhibition at 1 mM | Rhodococcus erythropolis | |
Al3+ | 21.6% residual activity at 1 mM | Rhodococcus erythropolis | |
Ba2+ | 85.8% residual activity at 1 mM | Rhodococcus erythropolis | |
Ca2+ | 89.3% residual activity at 1 mM | Rhodococcus erythropolis | |
Co2+ | 79.5% residual activity at 1 mM | Rhodococcus erythropolis | |
Cu2+ | complete inhibition at 1 mM | Rhodococcus erythropolis | |
DMSO | 8.4% residual activity at 40% (v/v) | Rhodococcus erythropolis | |
EDTA | - |
Rhodococcus erythropolis | |
ethanol | 3.7% residual activity at 10% (v/v) | Rhodococcus erythropolis | |
Fe2+ | complete inhibition at 1 mM | Rhodococcus erythropolis | |
methanol | 14% residual activity at 10% (v/v) | Rhodococcus erythropolis | |
Mn2+ | 81.1% residual activity at 1 mM | Rhodococcus erythropolis | |
Zn2+ | 3.4% residual activity at 1 mM | Rhodococcus erythropolis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
NADH | with diacetyl as cosubstrate, at pH 10.0 and 45°C | Rhodococcus erythropolis | |
0.1 | - |
diacetyl | at pH 10.0 and 45°C | Rhodococcus erythropolis | |
0.58 | - |
(2R,3R)-butane-2,3-diol | at pH 6.5 and 55°C | Rhodococcus erythropolis | |
1.04 | - |
NAD+ | with (2R,3R)-butane-2,3-diol as cosubstrate, at pH 6.5 and 55°C | Rhodococcus erythropolis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 138.9% activity at 1 mM | Rhodococcus erythropolis | |
additional information | Na+ and Mg2+ cause no significant activation | Rhodococcus erythropolis | |
Zn2+ | a zinc-containing alcohol dehydrogenase | Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
- |
Rhodococcus erythropolis |
45000 | - |
gel filtration | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3R)-butane-2,3-diol + NAD+ | Rhodococcus erythropolis | - |
(R)-acetoin + NADH + H+ | - |
r | |
(R)-acetoin + NADH + H+ | Rhodococcus erythropolis | the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0 | (2R,3R)-butane-2,3-diol + NAD+ | - |
r | |
(R)-acetoin + NADH + H+ | Rhodococcus erythropolis WZ010 | the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0 | (2R,3R)-butane-2,3-diol + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | A0A0E4A9D6 | - |
- |
Rhodococcus erythropolis WZ010 | A0A0E4A9D6 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography and Phenyl-Sepharose column chromatography | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3R)-butane-2,3-diol + NAD+ | - |
Rhodococcus erythropolis | (R)-acetoin + NADH + H+ | - |
r | |
(2R,3R)-butane-2,3-diol + NAD+ | 69.5% activity compared to (2R,3R)-butane-2,3-diol | Rhodococcus erythropolis | ? | - |
? | |
(R)-1-phenyl-1,2-ethanediol + NAD+ | 32.2% activity compared to (2R,3R)-butane-2,3-diol | Rhodococcus erythropolis | ? | - |
? | |
(R)-acetoin + NADH + H+ | the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0 | Rhodococcus erythropolis | (2R,3R)-butane-2,3-diol + NAD+ | - |
r | |
(R)-acetoin + NADH + H+ | the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0 | Rhodococcus erythropolis WZ010 | (2R,3R)-butane-2,3-diol + NAD+ | - |
r | |
2,2,2-trifluoroacetophenone + NADH + H+ | 28% activity compared to (R)-acetoin | Rhodococcus erythropolis | ? | - |
? | |
2,2,2-trifluoroacetophenone + NADH + H+ | 28% activity compared to (R)-acetoin | Rhodococcus erythropolis WZ010 | ? | - |
? | |
2-hydroxyacetophenone + NADH + H+ | 8.7% activity compared to (R)-acetoin | Rhodococcus erythropolis | ? | - |
? | |
2-octanone + NADH + H+ | 9.3% activity compared to (R)-acetoin | Rhodococcus erythropolis | ? | - |
? | |
3-methyl-2-butenal + NADH + H+ | 16.7% activity compared to (R)-acetoin | Rhodococcus erythropolis | ? | - |
? | |
butane-1,3-diol + NAD+ | 14.7% activity compared to (2R,3R)-butane-2,3-diol | Rhodococcus erythropolis | ? | - |
? | |
diacetyl + NADH + H+ | 69.3% activity compared to (R)-acetoin | Rhodococcus erythropolis | (R)-acetoin + NAD+ | - |
r | |
diacetyl + NADH + H+ | 69.3% activity compared to (R)-acetoin | Rhodococcus erythropolis WZ010 | (R)-acetoin + NAD+ | - |
r | |
ethyl 4-chloro-3-hydroxybutyrate + NAD+ | 12.2% activity compared to (2R,3R)-butane-2,3-diol | Rhodococcus erythropolis | ? | - |
? | |
glycerol + NAD+ | 16.3% activity compared to (2R,3R)-butane-2,3-diol | Rhodococcus erythropolis | ? | - |
? | |
glycerol + NAD+ | 16.3% activity compared to (2R,3R)-butane-2,3-diol | Rhodococcus erythropolis WZ010 | ? | - |
? | |
additional information | no activity with acetoin plus NAD+ or (2S,3S)-butane-2,3-diol witrh NAD+, or (S)-1-phenyl-1,2-ethanediol plus NAD+. 5% or less activity with 2-butanol, ethyl lactate, isopropanol, 1-butanol, cyclohexanol, 2-pentanol, 2-octanol, acetophenone, and 4-hydroxy-2-butanone | Rhodococcus erythropolis | ? | - |
? | |
additional information | no activity with acetoin plus NAD+ or (2S,3S)-butane-2,3-diol witrh NAD+, or (S)-1-phenyl-1,2-ethanediol plus NAD+. 5% or less activity with 2-butanol, ethyl lactate, isopropanol, 1-butanol, cyclohexanol, 2-pentanol, 2-octanol, acetophenone, and 4-hydroxy-2-butanone | Rhodococcus erythropolis WZ010 | ? | - |
? | |
sodium lactate + NAD+ | 10.2% activity compared to (2R,3R)-butane-2,3-diol | Rhodococcus erythropolis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37180, calculated from amino acid sequence | Rhodococcus erythropolis |
monomer | 1 * 44000, His-tagged enzyme, SDS-PAGE | Rhodococcus erythropolis |
Synonyms | Comment | Organism |
---|---|---|
(2R,3R)-2,3-butanediol dehydrogenase | - |
Rhodococcus erythropolis |
BDH | - |
Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
for (2R,3R)-butane-2,3-diol oxidation | Rhodococcus erythropolis |
55 | - |
for acetoin reduction | Rhodococcus erythropolis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
after 6 h incubation at 45°C, the remaining activities for acetoin reduction and butane-2,3-diol oxidation are 49.7% and 43.5%, respectively | Rhodococcus erythropolis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.28 | - |
(2R,3R)-butane-2,3-diol | at pH 6.5 and 55°C | Rhodococcus erythropolis | |
5.73 | - |
NADH | with diacetyl as cosubstrate, at pH 10.0 and 45°C | Rhodococcus erythropolis | |
6.11 | - |
diacetyl | at pH 10.0 and 45°C | Rhodococcus erythropolis | |
10.27 | - |
NAD+ | with (2R,3R)-butane-2,3-diol as cosubstrate, at pH 6.5 and 55°C | Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
for acetoin reduction | Rhodococcus erythropolis |
10 | - |
for (2R,3R)-butane-2,3-diol oxidation | Rhodococcus erythropolis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | for the reduction of acetoin, more than 85% of its maximal activity is in the pH range of 6.5 to 7.0 | Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rhodococcus erythropolis | |
NADH | - |
Rhodococcus erythropolis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.379 | - |
(2R,3R)-butane-2,3-diol | at pH 6.5 and 55°C | Rhodococcus erythropolis | |
9.875 | - |
NAD+ | with (2R,3R)-butane-2,3-diol as cosubstrate, at pH 6.5 and 55°C | Rhodococcus erythropolis | |
61.1 | - |
diacetyl | at pH 10.0 and 45°C | Rhodococcus erythropolis | |
71.63 | - |
NADH | with diacetyl as cosubstrate, at pH 10.0 and 45°C | Rhodococcus erythropolis |