Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | - |
(2S,3S)-butane-2,3-diol | at pH 10.5 and 30°C | Corynebacterium glutamicum | |
0.23 | - |
(2S)-acetoin | at pH 10.5 and 30°C | Corynebacterium glutamicum | |
1.49 | - |
(R)-acetoin | at pH 7.0 and 30°C | Corynebacterium glutamicum | |
2.01 | - |
(R,R)-butane-2,3-diol | at pH 10.5 and 30°C | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-acetoin + NADH + H+ | Corynebacterium glutamicum | the enzyme is not absolutely specific for (S)-acetoin, though this is the preferred substrate | (2S,3S)-butane-2,3-diol + NAD+ | - |
? | |
(2S)-acetoin + NADH + H+ | Corynebacterium glutamicum ATCC 13032 | the enzyme is not absolutely specific for (S)-acetoin, though this is the preferred substrate | (2S,3S)-butane-2,3-diol + NAD+ | - |
? | |
(2S,3S)-butane-2,3-diol + NAD+ | Corynebacterium glutamicum | - |
(2S)-acetoin + NADH + H+ | - |
? | |
(2S,3S)-butane-2,3-diol + NAD+ | Corynebacterium glutamicum ATCC 13032 | - |
(2S)-acetoin + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
- |
- |
Corynebacterium glutamicum ATCC 13032 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-acetoin + NADH + H+ | the enzyme is not absolutely specific for (S)-acetoin, though this is the preferred substrate | Corynebacterium glutamicum | (2S,3S)-butane-2,3-diol + NAD+ | - |
? | |
(2S)-acetoin + NADH + H+ | the enzyme is not absolutely specific for (S)-acetoin, though this is the preferred substrate | Corynebacterium glutamicum ATCC 13032 | (2S,3S)-butane-2,3-diol + NAD+ | - |
? | |
(2S,3S)-butane-2,3-diol + NAD+ | - |
Corynebacterium glutamicum | (2S)-acetoin + NADH + H+ | - |
? | |
(2S,3S)-butane-2,3-diol + NAD+ | - |
Corynebacterium glutamicum ATCC 13032 | (2S)-acetoin + NADH + H+ | - |
? | |
(R)-acetoin + NADH + H+ | low activity | Corynebacterium glutamicum | (R,R)-butane-2,3-diol + NAD+ | - |
r | |
(R)-acetoin + NADH + H+ | low activity | Corynebacterium glutamicum ATCC 13032 | (R,R)-butane-2,3-diol + NAD+ | - |
r | |
(R,R)-butane-2,3-diol + NAD+ | - |
Corynebacterium glutamicum | (R)-acetoin + NADH + H+ | - |
r | |
(R,R)-butane-2,3-diol + NAD+ | - |
Corynebacterium glutamicum ATCC 13032 | (R)-acetoin + NADH + H+ | - |
r | |
diacetyl + NADH + H+ | - |
Corynebacterium glutamicum | (2S)-acetoin + NAD+ | - |
? | |
diacetyl + NADH + H+ | - |
Corynebacterium glutamicum ATCC 13032 | (2S)-acetoin + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2,3-butanediol dehydrogenase | - |
Corynebacterium glutamicum |
BDH | - |
Corynebacterium glutamicum |
ButA | - |
Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Corynebacterium glutamicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
(R,R)-butane-2,3-diol | at pH 10.5 and 30°C | Corynebacterium glutamicum | |
1.58 | - |
(R)-acetoin | at pH 7.0 and 30°C | Corynebacterium glutamicum | |
187.5 | - |
(2S,3S)-butane-2,3-diol | at pH 10.5 and 30°C | Corynebacterium glutamicum | |
221.2 | - |
(2S)-acetoin | at pH 10.5 and 30°C | Corynebacterium glutamicum |