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Literature summary for 1.1.1.4 extracted from

  • Rados, D.; Turner, D.L.; Catarino, T.; Hoffart, E.; Neves, A.R.; Eikmanns, B.J.; Blombach, B.; Santos, H.
    Stereospecificity of Corynebacterium glutamicum 2,3-butanediol dehydrogenase and implications for the stereochemical purity of bioproduced 2,3-butanediol (2016), Appl. Microbiol. Biotechnol., 100, 10573-10583.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
(2S,3S)-butane-2,3-diol at pH 10.5 and 30°C Corynebacterium glutamicum
0.23
-
(2S)-acetoin at pH 10.5 and 30°C Corynebacterium glutamicum
1.49
-
(R)-acetoin at pH 7.0 and 30°C Corynebacterium glutamicum
2.01
-
(R,R)-butane-2,3-diol at pH 10.5 and 30°C Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2S)-acetoin + NADH + H+ Corynebacterium glutamicum the enzyme is not absolutely specific for (S)-acetoin, though this is the preferred substrate (2S,3S)-butane-2,3-diol + NAD+
-
?
(2S)-acetoin + NADH + H+ Corynebacterium glutamicum ATCC 13032 the enzyme is not absolutely specific for (S)-acetoin, though this is the preferred substrate (2S,3S)-butane-2,3-diol + NAD+
-
?
(2S,3S)-butane-2,3-diol + NAD+ Corynebacterium glutamicum
-
(2S)-acetoin + NADH + H+
-
?
(2S,3S)-butane-2,3-diol + NAD+ Corynebacterium glutamicum ATCC 13032
-
(2S)-acetoin + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-
Corynebacterium glutamicum ATCC 13032
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-acetoin + NADH + H+ the enzyme is not absolutely specific for (S)-acetoin, though this is the preferred substrate Corynebacterium glutamicum (2S,3S)-butane-2,3-diol + NAD+
-
?
(2S)-acetoin + NADH + H+ the enzyme is not absolutely specific for (S)-acetoin, though this is the preferred substrate Corynebacterium glutamicum ATCC 13032 (2S,3S)-butane-2,3-diol + NAD+
-
?
(2S,3S)-butane-2,3-diol + NAD+
-
Corynebacterium glutamicum (2S)-acetoin + NADH + H+
-
?
(2S,3S)-butane-2,3-diol + NAD+
-
Corynebacterium glutamicum ATCC 13032 (2S)-acetoin + NADH + H+
-
?
(R)-acetoin + NADH + H+ low activity Corynebacterium glutamicum (R,R)-butane-2,3-diol + NAD+
-
r
(R)-acetoin + NADH + H+ low activity Corynebacterium glutamicum ATCC 13032 (R,R)-butane-2,3-diol + NAD+
-
r
(R,R)-butane-2,3-diol + NAD+
-
Corynebacterium glutamicum (R)-acetoin + NADH + H+
-
r
(R,R)-butane-2,3-diol + NAD+
-
Corynebacterium glutamicum ATCC 13032 (R)-acetoin + NADH + H+
-
r
diacetyl + NADH + H+
-
Corynebacterium glutamicum (2S)-acetoin + NAD+
-
?
diacetyl + NADH + H+
-
Corynebacterium glutamicum ATCC 13032 (2S)-acetoin + NAD+
-
?

Synonyms

Synonyms Comment Organism
2,3-butanediol dehydrogenase
-
Corynebacterium glutamicum
BDH
-
Corynebacterium glutamicum
ButA
-
Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Corynebacterium glutamicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.9
-
(R,R)-butane-2,3-diol at pH 10.5 and 30°C Corynebacterium glutamicum
1.58
-
(R)-acetoin at pH 7.0 and 30°C Corynebacterium glutamicum
187.5
-
(2S,3S)-butane-2,3-diol at pH 10.5 and 30°C Corynebacterium glutamicum
221.2
-
(2S)-acetoin at pH 10.5 and 30°C Corynebacterium glutamicum