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Literature summary for 1.1.1.4 extracted from

  • Takeda, M.; Muranushi, T.; Inagaki, S.; Nakao, T.; Motomatsu, S.; Suzuki, I.; Koizumi, J.
    Identification and characterization of a mycobacterial (2R,3R)-2,3-butanediol dehydrogenase (2011), Biosci. Biotechnol. Biochem., 75, 2384-2389.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene mbd1, DNA and amino acid sequence determination and analysis, phylogenetic analysis Mycobacterium sp.
phylogenetic analysis Mycolicibacterium smegmatis
phylogenetic analysis Mycolicibacterium vanbaalenii
phylogenetic analysis Mycolicibacterium gilvum

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
Mycobacterium sp.
Cu2+
-
Mycobacterium sp.
EDTA
-
Mycobacterium sp.
Hg2+
-
Mycobacterium sp.
Zn2+
-
Mycobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
(3R,3S)-acetoin pH 7.0, 30°C Mycobacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3R)-butane-2,3-diol + NAD+ Mycobacterium sp. preferred substrate (3R,3S)-acetoin + NADH + H+
-
r
rac-1,2-propanediol + NAD+ Mycobacterium sp.
-
hydroxyacetone + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium sp. F1T242 gene mbd1
-
Mycolicibacterium gilvum
-
-
-
Mycolicibacterium smegmatis
-
-
-
Mycolicibacterium vanbaalenii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by anion exchange and hydrophobic interaction chromatography, followed by ultrafiltration and native PAGE Mycobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3R)-butane-2,3-diol + NAD+ preferred substrate Mycobacterium sp. (3R,3S)-acetoin + NADH + H+
-
r
(2R,3R)-butane-2,3-diol + NAD+ preferred substrate, very low activity with the (S)-enantiomer Mycobacterium sp. (3R,3S)-acetoin + NADH + H+
-
r
(R)-1,2-propanediol + NAD+ 73% activity compared to (2R,3R)-butane-2,3-diol in the oxidation reaction, very low activity with the (S)-enantiomer Mycobacterium sp. hydroxyacetone + NADH + H+
-
?
1,2-hexanediol + NAD+ 50% activity compared to (2R,3R)-butane-2,3-diol in the oxidation reaction Mycobacterium sp. ? + NADH + H+
-
?
1,2-pentanediol + NAD+ 74% activity compared to (2R,3R)-butane-2,3-diol in the oxidation reaction Mycobacterium sp. ? + NADH + H+
-
?
meso-butane-2,3-diol + NAD+ 61% activity compared to (2R,3R)-butane-2,3-diol in the oxidation reaction Mycobacterium sp. acetoin + NADH + H+
-
r
additional information substrate specificity, overview. No or poor activity with ethanol, ethylene glycol, diethylene glycol, 1-propanol, 2-propanol, 1,3-propanediol, glycerol, dipropylene glycol, 1-butanol, 1,4-butanediol, 1,2,4-butanetriol, (2S,3S)-butane-2,3-diol, 1,5-pentanediol, 2,4-pentanediol, 3-methyl-1,5-pentandiol, and 1,2,6-hexantriol Mycobacterium sp. ?
-
?
rac-1,2-propanediol + NAD+
-
Mycobacterium sp. hydroxyacetone + NADH + H+
-
?
rac-1,2-propanediol + NAD+ 68% activity compared to (2R,3R)-butane-2,3-diol in the oxidation reaction Mycobacterium sp. hydroxyacetone + NADH + H+
-
?

Subunits

Subunits Comment Organism
? x * 36430, sequence calculation Mycobacterium sp.

Synonyms

Synonyms Comment Organism
(2R,3R)-2,3-butanediol dehydrogenase
-
Mycolicibacterium smegmatis
(2R,3R)-2,3-butanediol dehydrogenase
-
Mycolicibacterium vanbaalenii
(2R,3R)-2,3-butanediol dehydrogenase
-
Mycolicibacterium gilvum
(2R,3R)-2,3-butanediol dehydrogenase
-
Mycobacterium sp.
R,R-BD-DH
-
Mycolicibacterium smegmatis
R,R-BD-DH
-
Mycolicibacterium vanbaalenii
R,R-BD-DH
-
Mycolicibacterium gilvum
R,R-BD-DH
-
Mycobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Mycobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
rapid denaturation of the purified enzyme above 50°C Mycobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at, reduction reaction Mycobacterium sp.
7.5
-
assay at, oxidation reaction Mycobacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8.5 stable, rapid denaturation below pH 5.0 Mycobacterium sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Mycobacterium sp.
NADH
-
Mycobacterium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Mycobacterium sp. isoelectric focussing
-
4.9

General Information

General Information Comment Organism
evolution the enzyme has homology to the medium-chain dehydrogenases/reductases with preference for secondary alcohols, phylogenetic analysis Mycolicibacterium smegmatis
evolution the enzyme has homology to the medium-chain dehydrogenases/reductases with preference for secondary alcohols, phylogenetic analysis Mycolicibacterium vanbaalenii
evolution the enzyme has homology to the medium-chain dehydrogenases/reductases with preference for secondary alcohols, phylogenetic analysis Mycolicibacterium gilvum
evolution the enzyme has homology to the medium-chain dehydrogenases/reductases with preference for secondary alcohols, phylogenetic analysis Mycobacterium sp.