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Literature summary for 1.1.1.4 extracted from

  • Machielsen, R.; Uria, A.R.; Kengen, S.W.; van der Oost, J.
    Production and characterization of a thermostable alcohol dehydrogenase that belongs to the aldo-keto reductase uperfamily (2006), Appl. Environ. Microbiol., 72, 233-238.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.097
-
 NADH
-
 Pyrococcus furiosus
0.6
-
 NAD+
-
 Pyrococcus furiosus
6.5
-
 acetoin
-
 Pyrococcus furiosus
86.8
-
 2,3-Butanediol
-
 Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration Pyrococcus furiosus
32000
-
 1 * 32000, SDS-PAGE Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-butanediol + NAD+
-
 Pyrococcus furiosus acetoin + NADH + H+
-
 r
2-pentanone + NADH + H+
-
 Pyrococcus furiosus (S)-2-pentanol + NAD+
-
 ?
acetoin + NAD+ 18% of the activity with 2,3-butanediol, in the reverse reaction 150% of the activity with acetoin Pyrococcus furiosus diacetyl + NADH
-
 r
cyclohexanone + NADH 38% of the activity with acetoin Pyrococcus furiosus ?
-
 ?
dihydroxyacetone + NADH 36% of the activity with acetoin Pyrococcus furiosus ?
-
 ?
dihydroxyacetone phosphate + NADH 82% of the activity with acetoin Pyrococcus furiosus ?
-
 ?
pyruvic aldehyde + NADH 81% of the activity with acetoin Pyrococcus furiosus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 32000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
 catalytic activity increases up to Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 half-life: 130 min Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1
-
 reduction of ketones Pyrococcus furiosus
8.8
-
 oxidation of secondary alcohols Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
NAD+ can use NAD(H) and NADP(H) as cofactor, clear preference for NADH Pyrococcus furiosus
NADH can use NAD(H) and NADP(H) as cofactor, clear preference for NADH Pyrococcus furiosus
NADP+ 14% of the activity with NAD+ Pyrococcus furiosus